Effective Rat Lung Tumor Model for Stereotactic Body Radiation Therapy.

Authors:
Strahinja Stojadinovic, Ph.D.
Strahinja Stojadinovic, Ph.D.
UT Southwestern Medical Center
Associate Professor
Medical Physics
Dallas, TX | United States
Dr. Rolf A Brekken, PhD
Dr. Rolf A Brekken, PhD
UT Southwestern Medical Center
Professor
Dallas, Texas | United States

Radiat Res 2016 06 25;185(6):616-22. Epub 2016 May 25.

a ‚ÄČ Department of Radiation Oncology.

Stereotactic body radiation therapy (SBRT) has found an important role in the treatment of patients with non-small cell lung cancer, demonstrating improvements in dose distribution and even tumor cure rates, particularly for early-stage disease. Despite its emerging clinical efficacy, SBRT has primarily evolved due to advances in medical imaging and more accurate dose delivery, leaving a void in knowledge of the fundamental biological mechanisms underlying its activity. Thus, there is a critical need for the development of orthotropic animal models to further probe the biology associated with high-dose-per-fraction treatment typical of SBRT. We report here on an improved surgically based methodology for generating solitary intrapulmonary nodule tumors, which can be treated with simulated SBRT using the X-RAD 225Cx small animal irradiator and Small Animal RadioTherapy (SmART) Plan treatment system. Over 90% of rats developed solitary tumors in the right lung. Furthermore, the tumor response to radiation was monitored noninvasively via bioluminescence imaging (BLI), and complete ablation of tumor growth was achieved with 36 Gy (3 fractions of 12 Gy each). We report a reproducible, orthotopic, clinically relevant lung tumor model, which better mimics patient treatment regimens. This system can be utilized to further explore the underlying biological mechanisms relevant to SBRT and high-dose-per-fraction radiation exposure and to provide a useful model to explore the efficacy of radiation modifiers in the treatment of non-small cell lung cancer.

Download full-text PDF

Source
http://dx.doi.org/10.1667/RR14382.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966888PMC
June 2016
25 Reads
1 Citation
2.911 Impact Factor

Publication Analysis

Top Keywords

lung tumor
12
radiation therapy
8
body radiation
8
non-small cell
8
cell lung
8
biological mechanisms
8
lung cancer
8
stereotactic body
8
small animal
8
tumor model
8
sbrt
5
lung
5
treatment
5
tumor
5
radiation
5
report improved
4
improved surgically
4
relevant lung
4
model better
4
sbrt report
4

Similar Publications