Usefulness of Discarded Vitreous Samples from Routine Vitrectomy.

J Ophthalmol 2016 26;2016:2380764. Epub 2016 Apr 26.

RI-MUHC, Henry C. Witelson Ocular Pathology Laboratory, 1001 Boulevard Decarie, Block E, Lab No. E02.2389, Montreal, QC, Canada H4A 3J1; Department of Ophthalmology, McGill University, Montreal, QC, Canada.

Purpose. To describe the histopathological features of vitreous samples obtained after vitrectomy surgery from diabetic and nondiabetic patients. Methods. Vitreous specimens from 137 patients who underwent vitrectomy for different clinical conditions were analysed. All samples were centrifuged and each resulting pellet was fixed and processed as part of routine paraffin section histopathology. The histopathological features were categorized in a semiquantitative fashion. The samples from diabetic and nondiabetic patients were compared. Results. The 125 included patients (58 diabetic, 60% males) were aged 64.2 ± 13.9 years. The presence of hemorrhage, inflammatory cells, and histiocytes was significantly higher in the diabetic group (P < 0.001, P = 0.028, and P = 0.016, resp.), showing more vessels (P < 0.001) and ghost vessels (P = 0.049). The presence of inflammatory cells was the feature with the highest sensitivity for detecting diabetes mellitus (98%) and also the highest negative predictive value (89%). In the multivariate analysis, three variables emerged as independent significant predictors of diabetes in vitrectomy samples: hemorrhage, endothelial-lined vessels, and age (P < 0.001, P < 0.001, and P = 0.019, resp.). Conclusions. Different histopathological features can be found in vitreous samples from diabetic patients. Analysis of vitrectomy samples may serve as a tool for diabetes management.

Download full-text PDF

Source
http://dx.doi.org/10.1155/2016/2380764DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861774PMC
May 2016
3 Reads

Publication Analysis

Top Keywords

vitreous samples
12
histopathological features
12
diabetic nondiabetic
8
nondiabetic patients
8
inflammatory cells
8
vitrectomy samples
8
features vitreous
8
samples diabetic
8
samples
7
patients
5
diabetic
5
vitrectomy
5
predictive 89%
4
aged 642
4
patients compared
4
642 139
4
89% multivariate
4
semiquantitative fashion
4
years presence
4
highest negative
4

Similar Publications