Multilocus genotyping of Giardia duodenalis isolates from children in Oromia Special Zone, central Ethiopia.

Authors:
Teklu Wegayehu
Teklu Wegayehu
College of Natural Sciences
Dr Md Robiul Karim, PhD
Dr Md Robiul Karim, PhD
Bangabandhu Sheikh Mujibur Rahman Agricultural University
Assistant Professor
Cryptosporidium, Giardia, Microsporidium, Molecular epidemiology, Population genetics
Gazipur | Bangladesh
Junqiang Li
Junqiang Li
College of Animal Science and Veterinary Medicine
Gainesville | United States
Haileeyesus Adamu
Haileeyesus Adamu
Institute of Biotechnology
United States
Berhanu Erko
Berhanu Erko
Aklilu Lemma Institute of Pathobiology
Dr. Longxian Zhang, PhD
Dr. Longxian Zhang, PhD
Henan Agricultural University
Distinguished professor
Veterinary parasitology
Zhengzhou, Henan | China
Getachew Tilahun
Getachew Tilahun
Aklilu Lemma Institute of Pathobiology

BMC Microbiol 2016 May 21;16:89. Epub 2016 May 21.

Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.

Background: Giardia duodenalis is the etiologic agent of giardiasis in humans and other mammals worldwide. The burden of disease is high among children in developing countries where sanitation is inadequate. However, the epidemiology and genetic diversity of this parasite is poorly understood in Ethiopia. This study aimed to determine the prevalence and genetic diversity of G. duodenalis in asymptomatic children in Oromia Special Zone, central Ethiopia.

Results: A total of 286 fresh fecal specimens were collected from children and screened using microscopy and PCR. The prevalence of Giardia duodenalis was 10.8 % (31/286) and 16.8 % (48/286) as detected by microscopy and nested PCR, respectively. The infection rate by the study area, sex and age group difference was not significant (P > 0.05). Genotyping results showed that 22.9 % (11/48) of the isolates belonged to assemblage A while 77.1 % (37/48) belonged to assemblage B. Although double peaks were observed at the chromatogram level, no mixed assemblage or sub-assemblage infections were demonstrated. Isolates of assemblage A mostly belonged to the sub-assemblage AII and showed similarity with previously described isolates. However, there was great genetic variability within assemblage B that showed heterogeneous nucleotide positions. Fifteen of them were new genotypes: 5 at the triose phosphate isomerase (tpi), 2 at the β-giardin (bg), and 8 at the glutamate dehydrogenase (gdh) genes.

Conclusions: Giardia duodenalis mainly assemblage B infection was predominant among the asymptomatic children in the study area. The high polymorphism found in isolates of assemblage B warrants a more defining tool to discriminate assemblage B at the sub-assemblage level. The findings of the present study indicate that there is a need to carry out national screening programs aiming to detect asymptomatic infections to minimize the reservoir of the disease.

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http://dx.doi.org/10.1186/s12866-016-0706-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4875739PMC

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May 2016
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