Molecular survey of Enterocytozoon bieneusi in sheep and goats in China.

Authors:
Ke Shi
Ke Shi
West China Hospital
United States
Mengjie Li
Mengjie Li
Key Laboratory of Meat Processing and Quality Control
China
Xiaoxing Wang
Xiaoxing Wang
College of Animal Science and Veterinary Medicine
United States
Junqiang Li
Junqiang Li
College of Animal Science and Veterinary Medicine
Gainesville | United States
Dr Md Robiul Karim, PhD
Dr Md Robiul Karim, PhD
Bangabandhu Sheikh Mujibur Rahman Agricultural University
Assistant Professor
Cryptosporidium, Giardia, Microsporidium, Molecular epidemiology, Population genetics
Gazipur | Bangladesh
Rongjun Wang
Rongjun Wang
College of Animal Science and Veterinary Medicine
China
Dr. Longxian Zhang, PhD
Dr. Longxian Zhang, PhD
Henan Agricultural University
Distinguished professor
Veterinary parasitology
Zhengzhou, Henan | China
Fuchun Jian
Fuchun Jian
College of Animal Science and Veterinary Medicine
China

Parasit Vectors 2016 Jan 19;9:23. Epub 2016 Jan 19.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.

Background: Over recent years, several studies have conducted genotyping of Enterocytozoon bieneusi in various hosts worldwide using sequence analysis of the ribosomal internal transcribed spacer (ITS), however, relatively little is known about E. bieneusi in sheep and goats in China. Therefore this research was conducted to understand the prevalence and genotype distribution of E. bieneusi in farmed sheep and goats in China.

Results: A total of 1025 fecal specimens from farmed animals in various geographic areas were collected. Overall, PCR and sequence analysis of the ITS detected E. bieneusi in 34.4% (353/1025) of isolates; of which the prevalence in goats was 28.8% (176/611) and in sheep was 42.8% (177/414). Phylogenetic analysis of ITS sequences identified 42 genotypes (nine known and 33 novel ones). These consisted of four known genotypes (D, KIN-1, EbpC, and F) and 10 novel genotypes (CHG6, CHG7, CHG9, CHG19, CHG23, CHG25, CHS5 and CHS10-CHS12) which all belonged to the so-called zoonotic group 1. A further four known genotypes (CD6, COS-I, BEB6, and J) and 22 novel genotypes (CHG1-CHG3, CHG5, CHG8, CHG10-CHG14, CHG16-CHG18, CHG20, CHG22, CHG24, CHS3, CHS4 and CHS6-CHS9) formed a clade within the group 2. One novel genotype (CHG21) was clustered in the group 9 with the genotype CM4.

Conclusions: E. bieneusi is highly prevalent, widely distributed, and genetically diverse in Chinese farmed goats and sheep. Some of the genotypes identified are potentially zoonotic.

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http://dx.doi.org/10.1186/s13071-016-1304-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5024852PMC

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January 2016
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