DEVELOPMENT OF AN AFFORDABLE BIOMOLECULE COMBINATION FOR HUMAN KNEE MENISCUS CELL PROLIFERATION

Mamatha M Pillai, V Elakkiya, J Gopinathan, C Sabarinath, S Shanthakumari, K Santosh Sahanand, B K Dinakar Rai, Amitava Bhattacharyya, R Selvakumar

Overview

The present study investigates the impact of biomolecules (biotin, glucose, chondroitin sulphate, proline) as supplement, (individual and in combination) on primary human meniscus cell proliferation.

Summary

The biomolecules used in the study are cost effective and affordable, UCM can be used as an effective alternative to other expensive growth factors for tissue engineering studies.

Author Comments

Dr. Mamatha M Pillai, PhD
Dr. Mamatha M Pillai, PhD
Indian Institute of Technology, Bombay
Post Doctoral Fellow
Tissue engineering and regenerative medicine
Mumbai, Maharashtra | India
I hope you find this article thought-provoking.Dr. Mamatha M Pillai, PhD

Resources

SPRINGER
https://link.springer.com/article/10.1007%2Fs10616-015-9926-1

A combination of biomolecules enhances expression of E-cadherin and peroxisome proliferator-activated receptor gene leading to increased cell proliferation in primary human meniscal cells: an in vitro study.

Authors:
Dr. Mamatha M Pillai, PhD
Dr. Mamatha M Pillai, PhD
Indian Institute of Technology, Bombay
Post Doctoral Fellow
Tissue engineering and regenerative medicine
Mumbai, Maharashtra | India

Cytotechnology 2016 Oct 28;68(5):1747-61. Epub 2015 Oct 28.

Tissue Engineering Laboratory, PSG Institute of Advanced Studies, Coimbatore, 641004, India.

The present study investigates the impact of biomolecules (biotin, glucose, chondroitin sulphate, proline) as supplement, (individual and in combination) on primary human meniscus cell proliferation. Primary human meniscus cells isolated from patients undergoing meniscectomy were maintained in Dulbecco's Modified Eagle's Medium (DMEM). The isolated cells were treated with above mentioned biomolecules as individual (0-100 µg/ml) and in combinations, as a supplement to DMEM. Based on the individual biomolecule study, a unique combination of biomolecules (UCM) was finalized using one way ANOVA analysis. With the addition of UCM as supplement to DMEM, meniscal cells reached 100 % confluency within 4 days in 60 mm culture plate; whereas the cells in medium devoid of UCM, required 36 days for reaching confluency. The impact of UCM on cell viability, doubling time, histology, gene expression, biomarkers expression, extra cellular matrix synthesis, meniscus cell proliferation with respect to passages and donor's age were investigated. The gene expression studies for E-cadherin and peroxisome proliferator-activated receptor (PPAR∆) using RT-qPCR and immunohistochemical analysis for Ki67, CD34 and Vimentin confirmed that UCM has significant impact on cell proliferation. The extracellular collagen and glycosaminoglycan secretion in cells supplemented with UCM were found to increase by 31 and 37 fold respectively, when compared to control on the 4th day. The cell doubling time was reduced significantly when supplemented with UCM. The addition of UCM showed positive influence on different passages and age groups. Hence, this optimized UCM can be used as an effective supplement for meniscal tissue engineering.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10616-015-9926-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023548PMC
October 2016
1 Read
1.752 Impact Factor

Publication Analysis

Top Keywords

cell proliferation
16
primary human
12
ucm
9
meniscal cells
8
supplemented ucm
8
proliferation primary
8
human meniscus
8
supplement dmem
8
addition ucm
8
meniscus cell
8
proliferator-activated receptor
8
gene expression
8
doubling time
8
peroxisome proliferator-activated
8
e-cadherin peroxisome
8
combination biomolecules
8
cell
6
cells
6
compared control
4
4 days 60 mm
4

References

(Supplied by CrossRef)

DV Aswani et al.
Int J Pharm 2013

SH Ayo et al.
Am J Pathol 1990

BM Baker et al.
Osteoarthr Cartil 2009

A Barbul et al.
J Nutr 2008

HE Brink et al.
J Tissue Eng Regen Med 2009

JR Brown et al.
Lab Invest 2014

NI Chalisova et al.
Adv Gerontol 2013

S Chen et al.
Development 2013

M Demoor et al.
Biochim Biophys Acta 2014

D Fraser et al.
Am J Pathol 2003

U Freymann et al.
Acta Biomater 2012

Similar Publications