High-Throughput Microarray Incubations Using Multi-Well Chambers.

Methods Mol Biol 2016 ;1352:19-26

JPT Peptide Technologies GmbH, Volmerstr. 5, 12489, Berlin, Germany.

Peptide microarrays are ideal tools for a variety of applications ranging from epitope mapping to immune monitoring. Here we present a method for high-throughput screening of biological samples using only standard microtiter plate equipment. Parallel incubation of a large number of samples with a small library of peptides is enabled by printing multiple identical mini-arrays on one microarray slide and further combining four slides to yield an incubation frame possessing the dimensions of a 96-well microtiter plate. Applying conventional lab equipment such as ELISA washers, hundreds of samples can be processed in 1 day yielding approx. 200 data points in triplicates per sample.

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http://dx.doi.org/10.1007/978-1-4939-3037-1_2DOI Listing
August 2016

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