High-density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool.

Plant Biotechnol J 2016 May 15;14(5):1195-206. Epub 2015 Oct 15.

Life Sciences, University of Bristol, Bristol, UK.

In wheat, a lack of genetic diversity between breeding lines has been recognized as a significant block to future yield increases. Species belonging to bread wheat's secondary and tertiary gene pools harbour a much greater level of genetic variability, and are an important source of genes to broaden its genetic base. Introgression of novel genes from progenitors and related species has been widely employed to improve the agronomic characteristics of hexaploid wheat, but this approach has been hampered by a lack of markers that can be used to track introduced chromosome segments. Here, we describe the identification of a large number of single nucleotide polymorphisms that can be used to genotype hexaploid wheat and to identify and track introgressions from a variety of sources. We have validated these markers using an ultra-high-density Axiom(®) genotyping array to characterize a range of diploid, tetraploid and hexaploid wheat accessions and wheat relatives. To facilitate the use of these, both the markers and the associated sequence and genotype information have been made available through an interactive web site.

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http://dx.doi.org/10.1111/pbi.12485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4950041PMC
May 2016
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References

(Supplied by CrossRef)
Chromosome aberrations in wheat nullisomic-tetrasomic and ditelosomic lines
Devos et al.
Cereal Res. Comm. 1999

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