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Fibrin gels loaded with cisplatin and cisplatin-hyaluronate complexes tested in a subcutaneous human melanoma model.

Invest New Drugs 2015 Dec 7;33(6):1151-61. Epub 2015 Oct 7.

U.O.S. Biopolimeri e Proteomica, IRCCS AOU San Martino-IST, Genova, Italy.

Fibrin gels are attractive biomaterials for local delivery of a variety of agents, from drugs to proteins. Similarly, polymer-anticancer-drug conjugates and nanoparticles are emerging as potential candidates for cancer treatment. Combining these different approaches, we have studied the efficacy of fibrin gels loaded with cisplatin (DDP) and a complex of DDP with hyaluronate (DDP-HA) for tumor growth inhibition in a melanoma model. Loaded gels prepared at relatively high fibrinogen concentration (22 mg/ml) showed good in vitro antiproliferative activities, prolonged release of the anticancer drug, and a long persistence (10-15 days) in vivo when implanted subcutaneously (sc) in immunodeficient mice. Gels loaded with DDP or DDP-HA containing 1/3 or even 1/6 of their systemic dose (6 mg/kg) and positioned under the tumor mass in mice bearing a sc human SK-Mel-28 tumor showed an antitumor activity better than that of the original parent compound given intraperitoneally (ip). Moreover, in an additional experiment in vivo, fibrin gels loaded with N-trimethyl chitosan-based nanoparticles containing a DDP-HA complex were assayed, resulting in a further 8 % improvement of anticancer activity, with lesser adverse systemic toxic effects. Taken together, these results suggest that the combination of fibrin gels and drugs complexed with suitable macromolecules holds great promise for loco-regional anticancer therapy of melanoma and other surgically removable cancer types.

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December 2015
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Bogomolets National Medical University, Kyiv, Ukraine.

Mechanical damage to the peripheral nerve is a fairly common type of injury, which is characterized by a complex of long-term neurological disorders and require significant financial costs. The aim of this work is to evaluate the efficiency of sciatic nerve (SN) regeneration after neuroraphy using epineural suture (ES), polyethylene glycol hydrogel (PEG), and fibrin glue (FG). The studies were carried out on 30 white outbred male rats, which were divided into six experimental groups: Group №1: intact rats; Group №2: Sham operated; Group №3: complete transection of the SN; Group №4: nerve repair with ES; Group №5: nerve repair with PEG; Group №6: nerve repair with FG. Read More

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Photopatterned biomolecule immobilization to guide three-dimensional cell fate in natural protein-based hydrogels.

Proc Natl Acad Sci U S A 2021 Jan;118(4)

Department of Chemical Engineering, University of Washington, Seattle, WA 98105;

Hydrogel biomaterials derived from natural biopolymers (e.g., fibrin, collagen, decellularized extracellular matrix) are regularly utilized in three-dimensional (3D) cell culture and tissue engineering. Read More

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Comparative Studies of Fibrin-Based Engineered Vascular Tissues and Notch Signaling from Progenitor Cells.

ACS Biomater Sci Eng 2020 May 30;6(5):2696-2706. Epub 2020 Mar 30.

Department of Chemical and Biochemical Engineering, The University of Western Ontario, 1151 Richmond Street, London, Ontario N6A 5B9, Canada.

The main impetus of vascular tissue engineering is clinical translation, but an equally appealing and impactful use of engineered vascular tissues is as preclinical testing platforms for studying vascular disease and developing therapeutic drugs and understanding of physiologically relevant vascular biology. Developing model engineered tissues will aid in narrowing the significant knowledge gaps in functional tissue formation, which is regulated by intricate cell signaling in a three-dimensional space. In this study, we fabricated tubular engineered vascular tissues using cross-linked fibrinogen as a scaffold and nondifferentiated embryonic rat vascular smooth muscle cell line (A10 cells) and mouse embryonic multipotent mesenchymal progenitor cell line (10T1/2 cells) as model vascular cells. Read More

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Transparent PDMS Bioreactors for the Fabrication and Analysis of Multi-Layer Pre-vascularized Hydrogels Under Continuous Perfusion.

Front Bioeng Biotechnol 2020 23;8:568934. Epub 2020 Dec 23.

Clinic for Trauma Surgery, Orthopedics and Plastic Surgery, University Medical Center Göttingen, Göttingen, Germany.

Tissue engineering in combination with stem cell technology has the potential to revolutionize human healthcare. It aims at the generation of artificial tissues that can mimic the original with complex functions for medical applications. However, even the best current designs are limited in size, if the transport of nutrients and oxygen to the cells and the removal of cellular metabolites waste is mainly dependent on passive diffusion. Read More

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Controlled Strain of 3D Hydrogels under Live Microscopy Imaging.

J Vis Exp 2020 12 4(166). Epub 2020 Dec 4.

School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University; Center for the Physics and Chemistry of Living Systems, Tel-Aviv University;

External forces are an important factor in tissue formation, development, and maintenance. The effects of these forces are often studied using specialized in vitro stretching methods. Various available systems use 2D substrate-based stretchers, while the accessibility of 3D techniques to strain soft hydrogels, is more restricted. Read More

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December 2020