Messenger RNA expression profile of sleep-related genes in peripheral blood cells in patients with chronic kidney disease.

Clin Exp Nephrol 2016 Apr 20;20(2):218-25. Epub 2015 Sep 20.

Division of Nephrology, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa, 920-8641, Japan.

Background: Various sleep abnormalities, such as delayed sleep onset, frequent awakening and daytime sleepiness, deteriorate the quality of life in patients with chronic kidney disease (CKD), including those on haemodialysis (HD). Although there are some candidate causative molecules in the central nervous system, the contribution of peripheral blood cells (PBCs) remains unclear. In this study, we performed polysomnographic analysis in CKD patients and used PBCs to examine the expression of genes related to sleep and wakefulness states.

Methods: Polysomnographic analysis was performed in 9 CKD patients and 6 controls. Genes related to sleep and wakefulness were evaluated by RNA microarray in 19 subjects, including CKD patients and control subjects.

Results: Polysomnographic analysis revealed that the duration of the rapid eye movement (REM)/non-REM phases during total sleep time was different between CKD patients and healthy controls. In mRNA microarray evaluation, hierarchical clustering analysis showed different patterns of sleep-related gene expression in HD patients. mRNA expression levels of GABA receptor (GABBR2), noradrenaline receptor (ADRA1A), dopamine receptor (DRD1) and histamine receptor (HRH1) showed an inverse correlation with renal function. Moreover, the mRNA expression of orexin and its receptor (HCRTR1 and HCRTR2) was also inversely correlated with renal function.

Conclusion: These data indicate that the expression of sleep-related genes in PBCs of CKD patients may be associated with sleep abnormalities.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10157-015-1150-yDOI Listing
April 2016
28 Reads

Publication Analysis

Top Keywords

ckd patients
20
polysomnographic analysis
12
blood cells
8
peripheral blood
8
patients chronic
8
sleep abnormalities
8
kidney disease
8
chronic kidney
8
mrna expression
8
patients
8
genes sleep
8
sleep-related genes
8
sleep wakefulness
8
ckd
6
expression
6
sleep
6
receptor
5
rem/non-rem phases
4
movement rem/non-rem
4
sleep time
4

References

(Supplied by CrossRef)

JL Holley et al.
Am J Kidney Dis 1992

S Walker et al.
Am J Kidney Dis 1995

SW Williams et al.
Am J Nephrol 2002

WM McClellan et al.
J Clin Epidemiol 1991

MR Pressman et al.
Sleep Res 1995

N Eryavuz et al.
Int Urol Nephrol 2008

PJ Hanly et al.
N Engl J Med 2001

E Iliescu et al.
Nephrol Dial Transpl 2003

ML Unruh et al.
Clin J Am Soc Nephrol 2006

KP Parker et al.
Nephrol Dial Transpl 2005

Y Arana-Lechuga et al.
World J Biol Psychiatry 2008

Similar Publications