Development and validation of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of tigecycline in rat brain tissues.

Authors:
Chiedza F Munyeza
Chiedza F Munyeza
University of KwaZulu-Natal
Dr Adeola Shobo, PhD
Dr Adeola Shobo, PhD
McGill University
Dr
Montreal , McGill University | Canada
Sooraj Baijnath
Sooraj Baijnath
University of KwaZulu-Natal
South Africa
Dominika Bratkowska
Dominika Bratkowska
Universitat Rovira i Virgili
Spain
Linda A Bester
Linda A Bester
University of KwaZulu-Natal
Pietermaritzburg | South Africa

Biomed Chromatogr 2016 Jun 20;30(6):837-45. Epub 2015 Oct 20.

Catalysis and Peptide Research Unit, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.

Tigecycline (TIG), a derivative of minocycline, is the first in the novel class of glycylcyclines and is currently indicated for the treatment of complicated skin structure and intra-abdominal infections. A selective, accurate and reversed-phase high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the determination of TIG in rat brain tissues. Sample preparation was based on protein precipitation and solid phase extraction using Supel-Select HLB (30 mg/1 mL) cartridges. The samples were separated on a YMC Triart C18 column (150 mm x 3.0 mm. 3.0 µm) using gradient elution. Positive electrospray ionization (ESI+) was used for the detection mechanism with the multiple reaction monitoring (MRM) mode. The method was validated over the concentration range of 150-1200 ng/mL for rat brain tissue. The precision and accuracy for all brain analyses were within the acceptable limit. The mean extraction recovery in rat brain was 83.6%. This validated method was successfully applied to a pharmacokinetic study in female Sprague Dawley rats, which were given a dose of 25 mg/kg TIG intraperitoneally at various time-points. Copyright © 2015 John Wiley & Sons, Ltd.

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http://dx.doi.org/10.1002/bmc.3616DOI Listing
June 2016
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References

(Supplied by CrossRef)
Molecular mechanisms of antibiotic resistance
Blair et al.
Nature Reviews Microbiology 2015
Tigecycline for the treatment of multidrug-resistant Klebsiella pneumoniae meningitis
Dandache et al.
Infectious Diseases in Clinical Practice 2009

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