Genome-wide association links candidate genes to resistance to Plum Pox Virus in apricot (Prunus armeniaca).

New Phytol 2016 Jan 10;209(2):773-84. Epub 2015 Sep 10.

UMR 1332 Biologie du Fruit et Pathologie, Equipe de Virologie, INRA, Université de Bordeaux, CS20032, F-33882, Villenave d'Ornon, France.

In fruit tree species, many important traits have been characterized genetically by using single-family descent mapping in progenies segregating for the traits. However, most mapped loci have not been sufficiently resolved to the individual genes due to insufficient progeny sizes for high resolution mapping and the previous lack of whole-genome sequence resources of the study species. To address this problem for Plum Pox Virus (PPV) candidate resistance gene identification in Prunus species, we implemented a genome-wide association (GWA) approach in apricot. This study exploited the broad genetic diversity of the apricot (Prunus armeniaca) germplasm containing resistance to PPV, next-generation sequence-based genotyping, and the high-quality peach (Prunus persica) genome reference sequence for single nucleotide polymorphism (SNP) identification. The results of this GWA study validated previously reported PPV resistance quantitative trait loci (QTL) intervals, highlighted other potential resistance loci, and resolved each to a limited set of candidate genes for further study. This work substantiates the association genetics approach for resolution of QTL to candidate genes in apricot and suggests that this approach could simplify identification of other candidate genes for other marked trait intervals in this germplasm.

Download full-text PDF

Source
http://dx.doi.org/10.1111/nph.13627DOI Listing
January 2016
22 Reads

Publication Analysis

Top Keywords

candidate genes
16
loci resolved
8
pox virus
8
apricot prunus
8
prunus armeniaca
8
genome-wide association
8
plum pox
8
resistance
5
genes
5
candidate
5
trait loci
4
identification prunus
4
gene identification
4
resistance gene
4
species implemented
4
association gwa
4
gwa approach
4
approach apricot
4
resistance quantitative
4
implemented genome-wide
4

Similar Publications