Rapid, culture-independent, optical diagnostics of centrifugally captured bacteria from urine samples.

Katharina Wolf
Katharina Wolf
Knappschaft Eye Clinic Sulzbach
Wolfgang Pfister
Wolfgang Pfister
University Hospital of Jena
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Fraunhofer Project Centre at Dublin City University
Professor (Full)
microfluidics, Lab-on-a-Chip, hydrodynanmics, business development, project management, organisational leadership
Glasnevin, Dublin 9 | Ireland

Biomicrofluidics 2015 Jul 11;9(4):044118. Epub 2015 Aug 11.

Biomedical Diagnostics Institute, National Centre of Sensor Research, School of Physical Sciences, Dublin City University , Glasnevin, Dublin 9, Ireland.

This work presents a polymeric centrifugal microfluidic platform for the rapid and sensitive identification of bacteria directly from urine, thus eliminating time-consuming cultivation steps. This "Lab-on-a-Disc" platform utilizes the rotationally induced centrifugal field to efficiently capture bacteria directly from suspension within a glass-polymer hybrid chip. Once trapped in an array of small V-shaped structures, the bacteria are readily available for spectroscopic characterization, such as Raman spectroscopic fingerprinting, providing valuable information on the characteristics of the captured bacteria. Utilising fluorescence microscopy, quantification of the bacterial load has been achieved for concentrations above 2 × 10(-7) cells ml(-1) within a 4 μl sample. As a pilot application, we characterize urine samples from patients with urinary tract infections. Following minimal sample preparation, Raman spectra of the bacteria are recorded following centrifugal capture in stopped-flow sedimentation mode. Utilizing advanced analysis algorithms, including extended multiplicative scattering correction, high-quality Raman spectra of different pathogens, such as Escherichia coli or Enterococcus faecalis, are obtained from the analyzed patient samples. The whole procedure, including sample preparation, requires about 1 h to obtain a valuable result, marking a significant reduction in diagnosis time when compared to the 24 h and more typically required for standard microbiological methods. As this cost-efficient centrifugal cartridge can be operated using low-complexity, widely automated instrumentation, while providing valuable bacterial identification in urine samples in a greatly reduced time-period, our opto-microfluidic Lab-on-a-Disc device demonstrates great potential for next-generation patient diagnostics at the of point-of-care.

Download full-text PDF

http://dx.doi.org/10.1063/1.4928070DOI Listing

Still can't find the full text of the article?

Sign up to send a request to the authors directly.
July 2015
18 Reads
4 PubMed Central Citations(source)
3.36 Impact Factor

Publication Analysis

Top Keywords

urine samples
raman spectra
bacteria directly
providing valuable
captured bacteria
sample preparation
mode utilizing
sedimentation mode
capture stopped-flow
bacteria recorded
spectra bacteria
recorded centrifugal
centrifugal capture
utilizing advanced
stopped-flow sedimentation
analysis algorithms
correction high-quality
high-quality raman
spectra pathogens


(Supplied by CrossRef)

Similar Publications

Sonication of orthopaedic implants: A valuable technique for diagnosis of prosthetic joint infections.

J Microbiol Methods 2018 Mar 31;146:51-54. Epub 2018 Jan 31.

Department of Microbiology, All India Institute of Medical Sciences (AIIMS), New Delhi, India. Electronic address:

Introduction: Accurate and prompt microbiological diagnosis of prosthetic joint infection (PJI) is crucial for successful antimicrobial treatment. Studies have shown the diagnostic utility of sonication of explanted implants in total joint arthroplasty but all did not use consensus statements for defining PJI. We evaluated the diagnostic utility of culture of samples obtained by sonication of explanted implants compared with periprosthetic tissue cultures (PTC) for the diagnosis of PJI using Musculoskeletal Infection Society (MSIS) consensus criteria. Read More

View Article
March 2018
8 Reads
2.03 Impact Factor

Contribution of ultrasound in the study of ovarian fibrothecomas: a series of 47 cases.

Tunis Med 2017 Jan;95(1):29-36

Introduction: Ovarian fibro-thecoma are rare presenting 1 to 4, 7%of ovarian organictumors. These tumors are of stromal origin and contain varied proportion of fusiform connective tissue cells and theca cells. They mainly affect menopausal or perimenopausal women. Read More

View Article
January 2017
3 Reads

Alteration of membrane integrity and respiratory function of brain mitochondria in the rats chronically exposed to a low dose of acetamiprid.

Environ Sci Pollut Res Int 2017 Oct 10;24(28):22258-22264. Epub 2017 Aug 10.

Laboratory of Food Neurotoxicology and Bioactivity, University of Metz, Metz, France.

The pesticides are used in several fields of agriculture and farms to protect crops against harmful insects and herbs. The increased and uncontrolled use of these pollutants is very hazardous for the population health. Consumption of contaminated food matrices with these pesticides could impair the cell integrity and its molecular function. Read More

View Article
October 2017
26 Reads
2.83 Impact Factor

Frequency and predictors of the lupus low disease activity state in a multi-national and multi-ethnic cohort.

Arthritis Res Ther 2016 11 9;18(1):260. Epub 2016 Nov 9.

The University of Melbourne, Melbourne, Australia.

Background: Systemic lupus erythematosus (SLE) is a chronic heterogeneous disease with considerable burden from disease activity and damage. A novel clinical treatment target in the form of the lupus low disease activity state (LLDAS) has been recently reported, with retrospective validation showing that time spent in LLDAS translates to reduced damage accrual. The objectives of this study were to describe the frequency and identify the predictors of attaining LLDAS in a large multinational cohort of patients with SLE. Read More

View Article
November 2016
31 Reads
4 PubMed Central Citations(source)
3.75 Impact Factor

Fasciola hepatica in snails collected from water-dropwort fields using PCR.

Korean J Parasitol 2014 Dec 23;52(6):645-52. Epub 2014 Dec 23.

Department of Infection Biology, Chungnam National University School of Medicine, Daejeon 301-131, Korea.

Fasciola hepatica is a trematode that causes zoonosis mainly in cattle and sheep and occasionally in humans. Fascioliasis has been reported in Korea; however, determining F. hepatica infection in snails has not been done recently. Read More

View Article
December 2014
17 Reads
7 PubMed Central Citations(source)
0.96 Impact Factor