Exposure to fluorescent light triggers down regulation of genes involved with mitotic progression in Xiphophorus skin.

Comp Biochem Physiol C Toxicol Pharmacol 2015 Dec 1;178:93-103. Epub 2015 Sep 1.

Molecular Bioscience Research Group &Xiphophorus Genetic Stock Center, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX 78666, United States. Electronic address:

We report RNA-Seq results from skin of X. maculatus Jp 163 B after exposure to various doses of "cool white" fluorescent light (FL). We show that FL exposure incites a genetic transcriptional response in skin nearly as great as observed for UVB exposure; however, the gene sets modulated due to exposure to the two light sources are quite different. Known light responsive genes involved in maintaining circadian cycling (e.g., clock, cry2a, cry1b, per1b, per2, per3, and arntl1a) exhibited expected shifts in transcriptional expression upon FL exposure. Exposure to FL also resulted in down-regulated transcription of many genes involved with cell cycle progression (e.g., cdc20, cdc45, cdca7b, plk1, cdk1, ccnb-3, and cdca7a) and chromosome segregation (e.g., cenpe, cenpf, cenpi, cenpk, cenpo, cenpp, and cenpu; cep70; knstrm, kntc, mcm2, mcm5; smc2). In addition, several DNA replication and recombination repair genes (e.g., pola1, pole, rec52, rad54l, rpa1, and parpbp) exhibit reduced expression in FL exposed X. maculatus skin. Some genes down modulated by FL are known to be associated with DNA repair and human diseases (e.g., atm2, brip1, fanc1, fancl, and xrcc4). The overall suppression of genes involved with mitotic progression in the skin of adult fish is consistent with entry into the light phase of the circadian cycle. Current efforts are aimed at determining specific wavelengths that may lead to differential expression among the many genes affected by fluorescent light exposure.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbpc.2015.08.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662871PMC
December 2015
3 Reads

Publication Analysis

Top Keywords

genes involved
16
fluorescent light
12
involved mitotic
8
mitotic progression
8
exposure
8
light exposure
8
genes
7
light
6
skin
5
progression cdc20
4
fanc1 fancl
4
xrcc4 suppression
4
cdc20 cdc45
4
plk1 cdk1
4
cdca7b plk1
4
fancl xrcc4
4
cdc45 cdca7b
4
ccnb-3 cdca7a
4
atm2 brip1
4
segregation cenpe
4

Similar Publications