Trans-Reactivation: A New Epigenetic Phenomenon Underlying Transcriptional Reactivation of Silenced Genes.

PLoS Genet 2015 Aug 20;11(8):e1005444. Epub 2015 Aug 20.

Dulbecco Telethon Institute, Palermo, Italy; Università degli Studi di Palermo, Dipartimento STEBICEF-Sezione Biologia Cellulare, Palermo, Italy.

In order to study the role played by cellular RNA pools produced by homologous genomic loci in defining the transcriptional state of a silenced gene, we tested the effect of non-functional alleles of the white gene in the presence of a functional copy of white, silenced by heterochromatin. We found that non-functional alleles of white, unable to produce a coding transcript, could reactivate in trans the expression of a wild type copy of the same gene silenced by heterochromatin. This new epigenetic phenomenon of transcriptional trans-reactivation is heritable, relies on the presence of homologous RNA's and is affected by mutations in genes involved in post-transcriptional gene silencing. Our data suggest a general new unexpected level of gene expression control mediated by homologous RNA molecules in the context of heterochromatic genes.

Download full-text PDF

Source
https://dx.plos.org/10.1371/journal.pgen.1005444
Publisher Site
http://dx.doi.org/10.1371/journal.pgen.1005444DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546373PMC
August 2015
79 Reads

Publication Analysis

Top Keywords

silenced heterochromatin
8
non-functional alleles
8
epigenetic phenomenon
8
alleles white
8
gene
5
genes involved
4
white gene
4
gene presence
4
presence functional
4
level gene
4
heterochromatin epigenetic
4
tested non-functional
4
silenced gene
4
state silenced
4
transcriptional state
4
gene expression
4
gene tested
4
gene silenced
4
transcriptional trans-reactivation
4
phenomenon transcriptional
4

References

(Supplied by CrossRef)
The eukaryotic genome as an RNA machine
PP Amaral et al.
Science 2008
Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression
AM Khalil et al.
Proceedings of the National Academy of Sciences of the United States of America 2009
RNA regulation of epigenetic processes
JS Mattick et al.
BioEssays: news and reviews in molecular, cellular and developmental biology 2009
RNA meets chromatin
E Bernstein et al.
Genes & development 2005

Similar Publications