Evidence for the presence of clofazimine and its distribution in the healthy mouse brain.

Authors:
Sooraj Baijnath
Sooraj Baijnath
University of KwaZulu-Natal
South Africa
Dr Adeola Shobo, PhD
Dr Adeola Shobo, PhD
McGill University
Dr
Montreal , McGill University | Canada
Chivonne Moodley
Chivonne Moodley
National Institute for Communicable Diseases of the National Health Laboratory Service
South Africa
John Adamson
John Adamson
KwaZulu-Natal Research Institute for Tuberculosis and HIV
Bongani Ngcobo
Bongani Ngcobo
KwaZulu-Natal Research Institute for Tuberculosis and HIV
Linda A Bester
Linda A Bester
University of KwaZulu-Natal
Pietermaritzburg | South Africa
Sanil Singh
Sanil Singh
University of KwaZulu-Natal
South Africa

J Mol Histol 2015 Oct 25;46(4-5):439-42. Epub 2015 Jul 25.

Catalysis and Peptide Research Unit, School of Pharmacy and Pharmacology, University of KwaZulu-Natal, Westville Campus, Durban, South Africa.

This is the first report of clofazimine (CFZ) penetration and distribution in normal mouse brain. Mice were administered 25 mg/kg CFZ or 100 mg/kg CFZ orally, daily for 2 weeks. Animals were sacrificed and blood and brain tissues were harvested. Liquid chromatography tandem mass spectrometry showed high concentrations of CFZ in homogenized brain, with 100 mg/kg dose having significantly higher concentration than 25 mg/kg. Matrix-assisted laser desorption/ionization spectrometric imaging of brain sections showed widespread tissue distribution of CFZ. Our results show dose dependent localization in brain.

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http://dx.doi.org/10.1007/s10735-015-9634-3DOI Listing
October 2015
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