Tissue distribution of pretomanid in rat brain via mass spectrometry imaging.

Authors:
Dr Adeola Shobo, PhD
Dr Adeola Shobo, PhD
McGill University
Dr
Montreal , McGill University | Canada
Dominika Bratkowska
Dominika Bratkowska
Universitat Rovira i Virgili
Spain
Sooraj Baijnath
Sooraj Baijnath
University of KwaZulu-Natal
South Africa
Anou M Somboro
Anou M Somboro
School of Health Sciences
Linda A Bester
Linda A Bester
University of KwaZulu-Natal
Pietermaritzburg | South Africa
Tricia Naicker
Tricia Naicker
School of Health Sciences

Xenobiotica 2016 24;46(3):247-52. Epub 2015 Jul 24.

a School of Pharmacy and Pharmacology, University of KwaZulu-Natal , Durban , South Africa and.

1. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) combines the sensitivity and selectivity of mass spectrometry with spatial analysis to provide a new dimension for histological analyses of the distribution of drugs in tissue. Pretomanid is a pro-drug belonging to a class of antibiotics known as nitroimidizoles, which have been proven to be active under hypoxic conditions and to the best of our knowledge there have been no studies investigating the distribution and localisation of this class of compounds in the brain using MALDI MSI. 2. Herein, we report on the distribution of pretomanid in the healthy rat brain after intraperitoneal administration (20 mg/kg) using MALDI MSI. Our findings showed that the drug localises in specific compartments of the rat brain viz. the corpus callosum, a dense network of neurons connecting left and right cerebral hemispheres. 3. This study proves that MALDI MSI technique has great potential for mapping the pretomanid distribution in uninfected tissue samples, without the need for molecular labelling.

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http://dx.doi.org/10.3109/00498254.2015.1067935DOI Listing
October 2016
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References

(Supplied by CrossRef)
Article in Int J Mass Spectrom
Heeren R et al.
Int J Mass Spectrom 2014

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