BMC Biophys 2014 9;7. Epub 2014 Sep 9.
Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, D-78457, Germany.
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BMC Biotechnol 2009 Apr 23;9:39. Epub 2009 Apr 23.
Lehrstuhl Molekulare Toxikologie, Fachbereich Biologie, Universität Konstanz, Konstanz, Germany.
Background: Formation and repair of DNA single-strand breaks are important parameters in the assessment of DNA damage and repair occurring in live cells. The 'Fluorimetric Detection of Alkaline DNA Unwinding (FADU)' method [Birnboim HC, Jevcak JJ. Cancer Res (1981) 41:1889-1892] is a sensitive procedure to quantify DNA strand breaks, yet it is very tedious to perform. Read More
ALTEX 2011 ;28(4):295-303
Molecular Toxicology Group, Department of Biology, University of Konstanz, Germany.
Genotoxicity tests are essential to identify compounds that have a potential to compromise not only the environment but also human and animal health, including compounds that increase the risk of cancer. At present, no single test is capable of detecting all types of genotoxic effects; therefore a battery of in vitro and, if positive, in vivo tests is necessary to determine the genotoxicity of a substance. However, the respective specificities of current assays have been criticized for their high percentage of false positive results. Read More
Toxicology 2013 Aug 21;310:15-21. Epub 2013 May 21.
University of Konstanz, Molecular Toxicology Group, Department of Biology, D-78457 Konstanz, Germany.
The oxidation of guanine to 8-oxo-2'-deoxyguanosine (8-oxo-dG) is one of the most abundant and best studied oxidative DNA lesions and is commonly used as a biomarker for oxidative stress. Over the last decades, various methods for the detection of DNA oxidation products have been established and optimized. However, some of them lack sensitivity or are prone to artifact formation, while others are time-consuming, which hampers their application in screening approaches. Read More
Methods Mol Biol 2017 ;1456:237-242
Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, AB, Canada, T1K 3M4.
DNA strand breaks arise from normal cellular processes such as replication, transcription, and DNA repair as well as spontaneous DNA damage caused by cell metabolic activities. In addition, strand breaks occur due to direct or indirect DNA damage produced by various abiotic and biotic stresses. Strand breaks are among the most problematic DNA lesions because unrepaired strand breaks may lead to cell cycle arrest, gross chromosome rearrangements, or even cell death. Read More