Genome-wide functional genomic and transcriptomic analyses for genes regulating sensitivity to vorinostat.

Sci Data 2014 8;1:140017. Epub 2014 Jul 8.

Victorian Centre for Functional Genomics, The Peter MacCallum Cancer Centre , St Andrews Place, East Melbourne, Victoria 3002, Australia ; Sir Peter MacCallum Department of Oncology, The University of Melbourne , Parkville, Victoria 3052, Australia.

Identification of mechanisms of resistance to histone deacetylase inhibitors, such as vorinostat, is important in order to utilise these anticancer compounds more efficiently in the clinic. Here, we present a dataset containing multiple tiers of stringent siRNA screening for genes that when knocked down conferred sensitivity to vorinostat-induced cell death. We also present data from a miRNA overexpression screen for miRNAs contributing to vorinostat sensitivity. Furthermore, we provide transcriptomic analysis using massively parallel sequencing upon knockdown of 14 validated vorinostat-resistance genes. These datasets are suitable for analysis of genes and miRNAs involved in cell death in the presence and absence of vorinostat as well as computational biology approaches to identify gene regulatory networks.

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http://dx.doi.org/10.1038/sdata.2014.17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322586PMC
November 2015
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