Assessment of dendritic arborization in the dentate gyrus of the hippocampal region in mice.

Authors:
Dr Mehmet Akif Baktir, MD
Dr Mehmet Akif Baktir, MD
Stanford University School of Medicine
Visiting Researcher
Physiology
Turkey
Dr.  Cristy Phillips, PT, MSPT, SCCD, EdD
Dr. Cristy Phillips, PT, MSPT, SCCD, EdD
Arkansas State University
Assistant Professor
Rehabilitation
Jonesboro , AR | United States
Prof Ahmad Salehi, M.D., Ph.D.
Prof Ahmad Salehi, M.D., Ph.D.
Stanford University School of Medicine
Professor
Palo Alto, CA | United States

J Vis Exp 2015 Mar 31(97). Epub 2015 Mar 31.

VA Palo Alto Health Care System; Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine;

Dendritic arborization has been shown to be a reliable marker for examination of structural and functional integrity of neurons. Indeed, the complexity and extent of dendritic arborization correlates well with the synaptic plasticity in these cells. A reliable method for assessment of dendritic arborization is needed to characterize the deleterious effects of neurological disorders on these structures and to determine the effects of therapeutic interventions. However, quantification of these structures has proven to be a formidable task given their complex and dynamic nature. Fortunately, sophisticated imaging techniques can be paired with conventional staining methods to assess the state of dendritic arborization, providing a more reliable and expeditious means of assessment. Below is an example of how these imaging techniques were paired with staining methods to characterize the dendritic arborization in wild type mice. These complementary imaging methods can be used to qualitatively and quantitatively assess dendritic arborization that span a rather wide area within the hippocampal region.

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Source
http://dx.doi.org/10.3791/52371DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401398PMC
March 2015
3 Reads
2 Citations

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