Search our Database of Scientific Publications and Authors

I’m looking for a

    Details and Download Full Text PDF:
    Effect of different heterotrophic plate count methods on the estimation of the composition of the culturable microbial community.

    PeerJ 2015 31;3:e862. Epub 2015 Mar 31.
    AIT Austrian Institute of Technology GmbH, Bioresources Unit , Tulln , Austria.
    Heterotrophic plate counts (HPC) are routinely determined within the scope of water quality assessment. However, variable HPC methods with different cultivation parameters (i.e., temperature and media type) are applied, which could lead to significant effects in the outcome of the analysis. Therefore the effect of different HPC methods, according to DIN EN ISO 6222 and EPA, on the culturable microbial community composition was investigated by 16S rRNA gene sequence analysis and statistical evaluation was performed. The culturable community composition revealed significant effects assigned to temperature (p < 0.01), while for media type no statistical significance was observed. However, the abundance of certain detected bacteria was affected. Lower temperature (22 °C) showed the abundance of naturally occurring Pseudomonadaceae and Aeromonadaceae, whereas at high temperature (37 °C) numerous Enterobacteriaceae, Citrobacter spp. and Bacilli were identified. The highest biodiversity was detected at lower temperature, especially on R2A medium. These results indicate that different temperatures (low and high) should be included into HPC measurement and selection of media should, ideally, be adjusted to the monitored water source. Accordingly, it can be inferred that the HPC method is more suitable for continuous monitoring of the same water source than for single assessments of a water sample.
    PDF Download - Full Text Link
    ( Please be advised that this article is hosted on an external website not affiliated with
    Source Status ListingPossible

    Similar Publications

    Heterotrophic plate count methodology in the United States.
    Int J Food Microbiol 2004 May;92(3):307-15
    Water Supply and Water Resources Division, National Risk Management Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Cincinnati, OH 45268, USA.
    In the United States (US), the history of bacterial plate counting (BPC) methods used for water can be traced largely through Standard Methods for the Examination of Water and Wastewater (Standard Methods). The bacterial count method has evolved from the original Standard Methods (1st edition, 1905) plate count which used nutrient gelatin and incubation at 20 degrees C for 48 h, to the HPC method options in the latest edition of Standard Methods that provide greater flexibility of application, depending on the data needs of the water analyst. The use of agar-agar as a gelling agent, replacing gelatin, allowed the use of higher incubation temperatures and resulted in the "body temperature count" (37 degrees C) found in the 3rd through the 8th edition of Standard Methods. Read More
    Detection and characterization of filterable heterotrophic bacteria from rural groundwater supplies.
    Lett Appl Microbiol 2001 Apr;32(4):268-72
    Division of Plant and Soil Sciences, West Virginia University, Morgantown 26506-6057, USA.
    Aims: The chemical/physical environment of groundwater may contribute to the existence of a subpopulation of small-sized bacteria (filterable bacteria) that fails to be trapped on conventional 0.45 microm-pore-size membrane filters during routine bacteriological water quality analyses. Efforts were directed to determining an efficient recovery method for detection of such cells. Read More
    Biofilm bacterial communities and abundance in a full-scale drinking water distribution system in Shanghai.
    J Water Health 2010 Sep 9;8(3):593-600. Epub 2010 Mar 9.
    Laboratory of Water Science & Technology, School of Life Science & Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China.
    Community diversity and abundance of biofilms from a full-scale drinking water distribution system in Shanghai were characterized by denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA sequences and heterotrophic plate count (HPC), respectively. Bacteria affiliated to the Beta- and Gamma-Proteobacteria were dominating in both in-situ and HPC-culturable bacterial communities. Other bacteria present included members of Alphaproteobacteria, Bacteroides, Actinobacteria, Nitrospirae and Firmicutes. Read More
    Eubacterial 16S-rDNA amplicon profiling: a rapid technique for comparison and differentiation of heterotrophic plate count communities from drinking water.
    Int J Food Microbiol 2004 May;92(3):333-45
    Applied Microbial Biochemistry and Gene Technology Group, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9-166, A-1060 Vienna, Austria.
    Determination of the heterotrophic plate count (HPC) is commonly used as a surrogate to assess the general microbial water quality in drinking water. For routine monitoring applications, the HPC is investigated in a quantitative way. However, qualitative data about the HPC bacterial community composition and/or population dynamics are required for particular situations. Read More