[Spectrum-effect relationship of Moutan cortex against lipopolysaccharide-induced acute lung injury].

Zhongguo Zhong Yao Za Zhi 2014 Nov;39(22):4389-93

This research is to study the relationship between HPLC fingerprints of Moutan Cortex, Paeoniae Radix Rubra and Paeoniae Radix Alba and their activity on lipopolysaccharide-induced acute lung injury. HPLC fingerprints of each extract of Moutan Cortex,Paeoniae Radix Rubra and Paeoniae Radix Alba were established by an optimized HPLC-MS method. The activities of all samples against protein and tumor necrosis a factor were tested by the model of lipopolysaccharide-induced acute lung injury. The possible relationship between HPLC-MS fingerprints and the activitieswere deduced by the Partial least squares regression analysis method. Samples were analyzed by HPLC-MS/MS to identify the major peaks. The results showed that each sample had some effect on acute lung injury. Four components with a lager contribution rate of efficacy were calculated by the research of spectrum-effect relationship. Moutan Cortex exhibited good activity on acute lung injury, and gallic acid, paeoniflorin, galloylpaeoniflorin and paeonol were the main effective components.

Download full-text PDF

Source
November 2014
6 Reads

Publication Analysis

Top Keywords

acute lung
20
lung injury
16
moutan cortex
12
paeoniae radix
12
lipopolysaccharide-induced acute
12
hplc fingerprints
8
relationship moutan
8
radix alba
8
rubra paeoniae
8
radix rubra
8
acute
5
lung
5
method samples
4
analysis method
4
samples analyzed
4
identify major
4
regression analysis
4
hplc-ms/ms identify
4
analyzed hplc-ms/ms
4
activitieswere deduced
4

Similar Publications