J Virol Methods 2015 Jun 17;218:40-5. Epub 2015 Mar 17.
University of Helsinki, Department of Virology, Haartmaninkatu 3, 00290 Helsinki, Finland; Helsinki University Hospital Laboratory Division, Haartmaninkatu 3, 00290 Helsinki, Finland.
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J Med Virol 2014 Dec 24;86(12):2102-6. Epub 2014 Jun 24.
Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, Japan.
Several publications concerning the methods of real-time PCR for human parvovirus B19 (B19V) have appeared and some case reports mention B19V DNA loads. However, no large-scale study quantitating levels of B19V DNA in common or representative B19V manifestations such as erythema infectiosum and aplastic crisis has been performed. Consequently, using the TaqMan PCR assay, the B19V load in a large sample of subjects with erythema infectiosum or aplastic crisis was quantitated. Read More
Mol Cell Probes 2017 Dec 30;36:50-57. Epub 2017 Aug 30.
Beijing Key Laboratory of Blood Safety and Supply Technologies & Blood Products and Substitute Laboratory, Beijing Institute of Transfusion Medicine, Beijing 100850, China. Electronic address:
Human parvovirus B19 (B19V) and human parvovirus 4 (PARV4) are two parvoviruses known to infect humans and transmit through blood and plasma derived medicinal products (PDMPs). Inactivation of the two parvoviruses has proven to be difficult and nucleic acid testing (NAT) would be an efficient means to exclude viruses. In this study, an internally controlled multiplex quantitative real-time PCR (qPCR) assay for B19V and PARV4 simultaneous detection and quantification was established and evaluated. Read More
Transfusion 2016 Feb 9;56(2):440-8. Epub 2015 Sep 9.
National Screening Laboratory, Sanquin Diagnostic Services, Amsterdam, the Netherlands.
Background: To meet European guidelines for plasma for fractionation, plasma fractionators have implemented parvovirus B19 (B19V) and hepatitis A virus (HAV) nucleic acid test (NAT) screening on test pools. In this study we evaluate recently developed in-house NAT assays for B19V DNA and HAV RNA. The B19V NAT was designed to target two different regions of the B19V genome. Read More
Virol J 2016 09 17;13(1):155. Epub 2016 Sep 17.
Laboratory for Viral Safety of National Centre of Biomedical Analysis, Beijing Institute of Transfusion Medicine, No. 27 Taiping road, Haidian District, Beijing, 100850, China.
Background: Human parvovirus B19 (B19V) is a frequent contaminant of blood and plasma-derived medicinal products. Three distinct genotypes of B19V have been identified. The distribution of the three B19V genotypes has been investigated in various regions or countries. Read More