Pubfacts - Scientific Publication Data
  • Categories
  • |
  • Journals
  • |
  • Authors
  • Login
  • Categories
  • Journals

Search Our Scientific Publications & Authors

Publications
  • Publications
  • Authors
find publications by category +
Translate page:

Masking autoprocessing of Clostridium difficile toxin A by the C-terminus combined repetitive oligo peptides.

Authors:
Yongrong Zhang Therwa Hamza Si Gao Hanping Feng

Biochem Biophys Res Commun 2015 Apr 26;459(2):259-263. Epub 2015 Feb 26.

Department of Microbial Pathogenesis, University of Maryland Dental School, Baltimore, MD 21201, USA. Electronic address:

Clostridium difficile toxin A and B (TcdA and TcdB) are the major virulence factors of the bacterium, both of which consist of two enzymatic domains: an effector glucosyltransferase domain (GTD) and a cysteine protease domain (CPD) responsible for autocleavage and release of GTD. Although the CPDs from both toxins share a similar structure and mechanism of hexakisphosphate (InsP6)-induced activation, TcdA is substantially less sensitive to the autocleavage as compared with TcdB. In this study, we provided evidence of inter-domain regulation of CPD activity of TcdA and its autoprocessing. The C-terminus combined repetitive oligo peptides (CROPs) of TcdA reduced the accessibility of TcdB CPD to its substrate in a chimeric toxin TxB-Ar, consequently blocking autoprocessing. Moreover, interference of antibodies with the CROPs of full-length TcdA efficiently enhanced its GTD release. In conclusion, by utilizing chimeric toxins and specific antibodies, we identified that the CROPs of TcdA plays a crucial role in controlling the InsP6-mediated activation of CPD and autocleavage of GTD. Our data provides insights on the molecular mode of action of the C. difficile toxins.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrc.2015.02.095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4426850PMC
April 2015

Publication Analysis

Top Keywords

oligo peptides
8
difficile toxin
8
crops tcda
8
repetitive oligo
8
combined repetitive
8
clostridium difficile
8
c-terminus combined
8
tcda
6
tcda plays
4
regulation cpd
4
study provided
4
cpd activity
4
evidence inter-domain
4
activity tcda
4
plays crucial
4
tcda autoprocessing
4
provided evidence
4
autoprocessing c-terminus
4
masking autoprocessing
4
inter-domain regulation
4

Keyword Occurance

Similar Publications

Clinical analysis of 2860 cases of diabetes in pregnancy: a single-center retrospective study.

Authors:
Jia Chen Zhenyu Wang Weizhen Wu Haixia Chen Caijuan Zhong Lixuan Liang Yingtao Li

BMC Pregnancy Childbirth 2022 May 18;22(1):418. Epub 2022 May 18.

Department of Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.

Background: To investigate the epidemiological, clinical characteristics and outcomes of diabetes in pregnancy (DIP).

Methods: This single-center, retrospective study included 16,974 pregnant women hospitalized during 2018-2019. Among them, 2860 DIP patients were grouped according to diabetes type, glycemic status, and insulin use. Read More

View Article and Full-Text PDF
May 2022
Similar Publications

Parathyroid Hormone-Related Changes of Bone Structure.

Authors:
M Kužma P Jackuliak Z Killinger J Payer

Physiol Res 2021 Nov;70(Suppl 1):S3-S11

5th department of Internal Medicine, Comenius University Faculty of Medicine, University Hospital Bratislava, Bratislava, Slovakia.

Parathyroid hormone (PTH) increases the release of serum calcium through osteoclasts, which leads to bone resorption. Primary, PTH stimulates osteoblasts leading to increase RANKL (receptor activator for nuclear factor kappa-B ligand) expression and thus differentiation of osteoclasts. In kidneys, PTH increases calcium and decrease phosphate reabsorption. Read More

View Article and Full-Text PDF
November 2021
Similar Publications

Guanidinium-Functionalized Flexible Azaproline Transporter for Efficient Intracellular Delivery of Proapoptotic Peptide and PDL1 Antisense Morpholino Oligo in Human Carcinoma Cells .

Authors:
Abhishek Gupta Shalini Gupta Ujjal Das Surajit Sinha

Bioconjug Chem 2022 May 29;33(5):907-917. Epub 2022 Apr 29.

School of Applied and Interdisciplinary Sciences, Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700032, West Bengal, India.

Cell-penetrating peptides (CPPs) are structurally diverse sophisticated tools endowed with high arginine content, amphipathicity, and well-adopted suitable secondary structures. Despite its capability of breaching the lipid barriers, CPP has major limitations such as metabolic instability, poor bioavailability, and reduced endosomal escape tendency, which are yet to be improved. In this context, we first have introduced a new class of cellular transporter having a guanidinium-functionalized δ-azaproline (δ-azp)-containing peptide where the δ-azp structurally resembles the "proline" amino acid having an additional "" at the δ-position. Read More

View Article and Full-Text PDF
May 2022
Similar Publications

Compact Peptoid Molecular Brushes for Nanoparticle Stabilization.

Authors:
Shih-Ting Wang Honghu Zhang Sunting Xuan Dmytro Nykypanchuk Yugang Zhang Guillaume Freychet Benjamin M Ocko Ronald N Zuckermann Nevena Todorova Oleg Gang

J Am Chem Soc 2022 May 22;144(18):8138-8152. Epub 2022 Apr 22.

Center for Functional Nanomaterials, Brookhaven National Laboratory, Brookhaven Avenue, Upton, New York 11973, United States.

Controlling the interfaces and interactions of colloidal nanoparticles (NPs) via tethered molecular moieties is crucial for NP applications in engineered nanomaterials, optics, catalysis, and nanomedicine. Despite a broad range of molecular types explored, there is a need for a flexible approach to rationally vary the chemistry and structure of these interfacial molecules for controlling NP stability in diverse environments, while maintaining a small size of the NP molecular shell. Here, we demonstrate that low-molecular-weight, bifunctional comb-shaped, and sequence-defined peptoids can effectively stabilize gold NPs (AuNPs). Read More

View Article and Full-Text PDF
May 2022
Similar Publications

Female Pelvic Conditions: Polycystic Ovary Syndrome.

Authors:
Lesley D Wilkinson Patricia H Brady Geneen T Gin Elizabeth Rosenblum

FP Essent 2022 04;515:26-31

Department of Family Medicine - University of California San Diego School of Medicine, 402 Dickinson St, San Diego, CA 92103.

It is estimated that polycystic ovary syndrome (PCOS) affects about 10% of women of reproductive age in the United States. Principal risk factors include obesity and a family history of PCOS. A diagnosis of PCOS should be considered in women with irregular or absent menstrual cycles, issues related to hyperandrogenism, or infertility. Read More

View Article and Full-Text PDF
April 2022
Similar Publications
}
© 2022 PubFacts.
  • About PubFacts
  • Privacy Policy
  • Sitemap