MultiPLX: automatic grouping and evaluation of PCR primers.

Methods Mol Biol 2015 ;1275:127-42

Department of Bioinformatics, University of Tartu, Tartu, Estonia.

In this chapter we describe MultiPLX-a tool for automatic grouping of PCR primers for multiplexed PCR. Both generic working principle and step-by-step practical procedures with examples are presented. MultiPLX performs grouping by calculating many important interaction levels between the different primer pairs and then distributes primer pairs to groups so that the strength of unwanted interactions is kept below user-defined compatibility level. In addition it can be used to select optimal primer pairs for multiplexing from list of candidates. MultiPLX can be downloaded from http://bioinfo.ut.ee/?page_id=167. Graphical web-based interface to most functions of MultiPLX is available at http://bioinfo.ut.ee/multiplx/.

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-4939-2365-6_9DOI Listing
December 2015
4 Reads

Publication Analysis

Top Keywords

primer pairs
12
automatic grouping
8
pcr primers
8
pairs groups
4
distributes primer
4
pairs distributes
4
groups strength
4
strength unwanted
4
interactions user-defined
4
unwanted interactions
4
examples presented
4
levels primer
4
performs grouping
4
multiplx performs
4
grouping calculating
4
calculating interaction
4
user-defined compatibility
4
multiplx
4
presented multiplx
4
level addition
4

Similar Publications