Procedia Comput Sci 2014 ;20:2295-2305
National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA, USA.
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BMC Bioinformatics 2012 May 17;13:102. Epub 2012 May 17.
Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB, Canada.
Background: Microarray data analysis has been the subject of extensive and ongoing pipeline development due to its complexity, the availability of several options at each analysis step, and the development of new analysis demands, including integration with new data sources. Bioinformatics pipelines are usually custom built for different applications, making them typically difficult to modify, extend and repurpose. Scientific workflow systems are intended to address these issues by providing general-purpose frameworks in which to develop and execute such pipelines. Read More
J Struct Biol 2012 Oct 27;180(1):154-64. Epub 2012 Jun 27.
National Center For Microscopy and Imaging Research, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0608, USA.
Electron tomography (ET) has been proven an essential technique for imaging the structure of cells beyond the range of the light microscope down to the molecular level. Large-field high-resolution views of biological specimens span more than four orders of magnitude in spatial scale, and, as a consequence, are rather difficult to generate directly. Various techniques have been developed towards generating those views, from increasing the sensor array size to implementing serial sectioning and montaging. Read More
Microscopy (Oxf) 2014 Nov;63 Suppl 1:i25
Advanced Microscope Systems Design Dept., Hitachi High-Technologies Corporation, 882, Ichige, Hitachinaka, Ibaraki, Japan.
The ultra-high voltage electron microscope (UHVEM) H-3000 with the world highest acceleration voltage of 3 MV can observe remarkable three dimensional microstructures of microns-thick samples. Acquiring a tilt series of electron tomography is laborious work and thus an automatic technique is highly desired. We proposed the Auto-Focus system using image Sharpness (AFS)[2,3] for UHVEM tomography tilt series acquisition. Read More
Methods Cell Biol 1999 ;61:81-111
Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany 12201-0509, USA.
Transmission electron microscopy produces images that are projections of the original object, with the consequence that features from different depths of the specimen overlap and give a confusing image. This problem is overcome by reconstructing the object in 3D from a series of 2D views using either serial thin section reconstruction or electron tomography. In the serial section approach, the series of 2D views is generated from images of successive serial sections cut thin enough to be effectively 2D slices of the specimen. Read More