Cell lineage analysis in human brain using endogenous retroelements.

Neuron 2015 Jan;85(1):49-59

Division of Genetics and Genomics, Manton Center for Orphan Disease Research, and Howard Hughes Medical Institute, Boston Children's Hospital, Boston, MA 02115, USA; Departments of Neurology and Pediatrics, Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Electronic address:

Somatic mutations occur during brain development and are increasingly implicated as a cause of neurogenetic disease. However, the patterns in which somatic mutations distribute in the human brain are unknown. We used high-coverage whole-genome sequencing of single neurons from a normal individual to identify spontaneous somatic mutations as clonal marks to track cell lineages in human brain. Somatic mutation analyses in >30 locations throughout the nervous system identified multiple lineages and sublineages of cells marked by different LINE-1 (L1) retrotransposition events and subsequent mutation of poly-A microsatellites within L1. One clone contained thousands of cells limited to the left middle frontal gyrus, whereas a second distinct clone contained millions of cells distributed over the entire left hemisphere. These patterns mirror known somatic mutation disorders of brain development and suggest that focally distributed mutations are also prevalent in normal brains. Single-cell analysis of somatic mutation enables tracing of cell lineage clones in human brain.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neuron.2014.12.028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4299461PMC
January 2015
25 Reads

Publication Analysis

Top Keywords

human brain
12
somatic mutations
12
somatic mutation
8
brain development
8
clone contained
8
cell lineage
8
brain
5
somatic
5
mutation disorders
4
>30 locations
4
locations nervous
4
mirror somatic
4
patterns mirror
4
lineages sublineages
4
multiple lineages
4
identified multiple
4
analyses >30
4
system identified
4
nervous system
4
mutation analyses
4

Similar Publications