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    Structure-based design and synthesis of a bivalent iminobiotin analog showing strong affinity toward a low immunogenic streptavidin mutant.
    Biosci Biotechnol Biochem 2015 3;79(4):640-2. Epub 2015 Jan 3.
    a Division of Applied Chemistry, Graduate School of Engineering , Osaka University , Suita , Japan.
    The streptavidin/biotin interaction has been widely used as a useful tool in research fields. For application to a pre-targeting system, we previously developed a streptavidin mutant that binds to an iminobiotin analog while abolishing affinity for natural biocytin. Here, we design a bivalent iminobiotin analog that shows 1000-fold higher affinity than before, and determine its crystal structure complexed with the mutant protein.

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