Zinc induces distinct changes in the metabolism of reactive oxygen and nitrogen species (ROS and RNS) in the roots of two Brassica species with different sensitivity to zinc stress.

Ann Bot 2015 Sep 22;116(4):613-25. Epub 2014 Dec 22.

Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary and.

Background And Aims: Zinc (Zn) is an essential micronutrient naturally present in soils, but anthropogenic activities can lead to accumulation in the environment and resulting damage to plants. Heavy metals such as Zn can induce oxidative stress and the generation of reactive oxygen and nitrogen species (ROS and RNS), which can reduce growth and yield in crop plants. This study assesses the interplay of these two families of molecules in order to evaluate the responses in roots of two Brassica species under high concentrations of Zn.

Methods: Nine-day-old hydroponically grown Brassica juncea (Indian mustard) and B. napus (oilseed rape) seedlings were treated with ZnSO4 (0, 50, 150 and 300 µm) for 7 d. Stress intensity was assessed through analyses of cell wall damage and cell viability. Biochemical and cellular techniques were used to measure key components of the metabolism of ROS and RNS including lipid peroxidation, enzymatic antioxidants, protein nitration and content of superoxide radical ([Formula: see text]), nitric oxide (NO) and peroxynitrite (ONOO(-)).

Key Results: Analysis of morphological root damage and alterations of microelement homeostasis indicate that B. juncea is more tolerant to Zn stress than B. napus. ROS and RNS parameters suggest that the oxidative components are predominant compared with the nitrosative components in the root system of both species.

Conclusions: The results indicate a clear relationship between ROS and RNS metabolism as a mechanism of response against stress caused by an excess of Zn. The oxidative stress components seem to be more dominant than the elements of the nitrosative stress in the root system of these two Brassica species.

Download full-text PDF

Source
http://dx.doi.org/10.1093/aob/mcu246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577986PMC
September 2015
20 Reads

Publication Analysis

Top Keywords

ros rns
20
brassica species
12
species ros
8
nitrogen species
8
oxygen nitrogen
8
roots brassica
8
oxidative stress
8
root system
8
reactive oxygen
8
stress
7
rns
5
species
5
ros
5
key components
4
components metabolism
4
metabolism ros
4
rns including
4
components
4
viability biochemical
4
biochemical cellular
4

References

(Supplied by CrossRef)

Similar Publications