Multi-locus analysis of Giardia duodenalis from nonhuman primates kept in zoos in China: geographical segregation and host-adaptation of assemblage B isolates.

Authors:
Dr Md Robiul Karim, PhD
Dr Md Robiul Karim, PhD
Bangabandhu Sheikh Mujibur Rahman Agricultural University
Assistant Professor
Cryptosporidium, Giardia, Microsporidium, Molecular epidemiology, Population genetics
Gazipur | Bangladesh
Rongjun Wang
Rongjun Wang
College of Animal Science and Veterinary Medicine
China
Fuchang Yu
Fuchang Yu
College of Animal Science and Veterinary Medicine
Gainesville | United States
Tongyi Li
Tongyi Li
Zhengzhou Zoo
Zhengzhou Shi | China
Haiju Dong
Haiju Dong
College of Animal Science and Veterinary Medicine
Gainesville | United States
Dezhong Li
Dezhong Li
Zhengzhou zoo
Zhengzhou | China
Dr. Longxian Zhang, PhD
Dr. Longxian Zhang, PhD
Henan Agricultural University
Distinguished professor
Veterinary parasitology
Zhengzhou, Henan | China
Junqiang Li
Junqiang Li
College of Animal Science and Veterinary Medicine
Gainesville | United States

Infect Genet Evol 2015 Mar 18;30:82-88. Epub 2014 Dec 18.

Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. Electronic address:

Only a few studies based on single locus characterization have been conducted on the molecular epidemiology of Giardia duodenalis in nonhuman primates (NHPs). The present study was conducted to examine the occurrence and genotype identity of G. duodenalis in NHPs based on multi-locus analysis of the small-subunit ribosomal RNA (SSU rRNA), triose phosphate isomerase (tpi), glutamate dehydrogenase (gdh), and beta-giardin (bg) genes. Fecal specimens were collected from 496 animals of 36 NHP species kept in seven zoos in China and screened for G. duodenalis by tpi-based PCR. G. duodenalis was detected in 92 (18.6%) specimens from 18 NHP species, belonging to assemblage A (n=4) and B (n=88). In positive NHP species, the infection rates ranged from 4.8% to 100%. In tpi sequence analysis, the assemblage A included subtypes A1, A2 and one novel subtype. Multi-locus analysis of the tpi, gdh, and bg genes detected 11 (8 known and 3 new), 6 (3 known and 3 new) and 9 (2 known and 7 new) subtypes in 88, 47 and 35 isolates in assemblage B, respectively. Thirty-two assemblage B isolates with data at all three loci yielded 15 multi-locus genotypes (MLGs), including 2 known and 13 new MLGs. Phylogenetic analysis of concatenated sequences of assemblage B showed that MLGs found here were genetically different from those of humans, NHPs, rabbit and guinea pig in Italy and Sweden. It further indicated that assemblage B isolates in ring-tailed lemurs and squirrel monkeys might be genetically different from those in other NHPs. These data suggest that NHPs are mainly infected with G. duodenalis assemblage B and there might be geographical segregation and host-adaptation in assemblage B in NHPs.

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http://dx.doi.org/10.1016/j.meegid.2014.12.013DOI Listing

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March 2015
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