Viral genome and antiviral drug sensitivity analysis of two patients from a family cluster caused by the influenza A(H7N9) virus in Zhejiang, China, 2013.

Int J Infect Dis 2014 Dec 13;29:254-8. Epub 2014 Nov 13.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310031, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China. Electronic address:

Objectives: In the winter of 2013, people were facing the risk of human-to-human transmission of the re-emerging influenza A(H7N9) virus. We report herein information on the clinical features of two patients from the same family infected with this virus, the genomic sequences of the viruses harbored, and antiviral drug sensitivity.

Methods: Clinical and epidemiological data of two patients from the same family were collected and analyzed. Sequencing was done for the viruses isolated from these two patients and one epidemiologically related chicken, and the sequences of the eight gene segments of the viruses were analyzed phylogenetically. The sensitivity of the viruses to antiviral drug treatment was determined by neuraminidase inhibitor susceptibility test.

Results: The two patients from one family cluster shared the same symptoms but had different outcomes, and had a strong epidemiological link. Three strains, two from these two patients and one from the chicken, were isolated. Genome sequencing and analyses of phylogenetic trees demonstrated that the two viruses were almost identical. We noted the presence of the PB2 E627K amino acid substitution that was not present in isolates from the first wave, as well as two new mutations in the NA gene and six in the PB2 gene. Drug sensitivity testing showed that the new isolates were resistant to oseltamivir but sensitive to peramivir.

Conclusions: The two patients from one family cluster were probable human-to-human transmission cases. The new isolates were sensitive to peramivir but showed reduced sensitivity to oseltamivir.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijid.2014.10.029DOI Listing
December 2014
62 Reads
3 Citations
2.330 Impact Factor

Publication Analysis

Top Keywords

patients family
20
antiviral drug
12
family cluster
12
human-to-human transmission
8
ah7n9 virus
8
drug sensitivity
8
influenza ah7n9
8
patients
7
family
5
viruses
5
symptoms outcomes
4
outcomes strong
4
strong epidemiological
4
shared symptoms
4
cluster shared
4
testresults patients
4
epidemiological link
4
susceptibility testresults
4
three strains
4
isolated genome
4

Similar Publications