High abundant protein removal from rodent blood for biomarker discovery.

Authors:
Prof. Dominik R Haudenschild, Ph.D.
Prof. Dominik R Haudenschild, Ph.D.
University of California Davis Medical Center
Professor
Arthritis Research
Sacramento, CA | United States
Angela Eldridge
Angela Eldridge
University of California Davis School of Medicine
Sacramento | United States
Pamela J Lein
Pamela J Lein
School of Veterinary Medicine
United States
Brett A Chromy
Brett A Chromy
Lawrence Livermore National Laboratory
Livermore | United States

Biochem Biophys Res Commun 2014 Dec;455(1-2):84-9

In order to realize the goal of stratified and/or personalized medicine in the clinic, significant advances in the field of biomarker discovery are necessary. Adding to the abundance of nucleic acid biomarkers being characterized, additional protein biomarkers will be needed to satisfy diverse clinical needs. An appropriate source for finding these biomarkers is within blood, as it contains tissue leakage factors as well as additional proteins that reside in blood that can be linked to the presence of disease. Unfortunately, high abundant proteins and complexity of the blood proteome present significant challenges for the discovery of protein biomarkers from blood. Animal models often enable the discovery of biomarkers that can later be translated to humans. Therefore, determining appropriate sample preparation of proteomic samples in rodent models is an important research goal. Here, we examined both mouse and rat blood samples (including both serum and plasma), for appropriate high abundant protein removal techniques for subsequent gel-based proteomic experiments. We assessed four methods of albumin removal: antibody-based affinity chromatography (MARS), Cibacron® Blue-based affinity depletion (SwellGel® Blue Albumin Removal Kit), protein-based affinity depletion (ProteaPrep Albumin Depletion Kit) and TCA/acetone precipitation. Albumin removal was quantified for each method and SDS-PAGE and 2-DE gels were used to quantify the number of protein spots obtained following albumin removal. Our results suggest that while all four approaches can effectively remove high abundant proteins, antibody-based affinity chromatography is superior to the other three methods.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S0006291X140178
Publisher Site
http://dx.doi.org/10.1016/j.bbrc.2014.09.137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309281PMC

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December 2014
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2 Citations
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