Elife 2014 Nov 17;3. Epub 2014 Nov 17.
Institute of Biochemistry, Department of Biology, ETH Zürich, Zürich, Switzerland.
Download full-text PDF
Curr Biol 2011 Jan;21(1):R17-8
Summary: Asymmetric cell division in unicellular organisms enables sequestration of senescence factors to specific subpopulations. Accumulation of autonomously replicating sequence (ARS) plasmids, which frequently emerge from recombination within the highly repetitive ribosomal DNA locus, is linked to limited replicative life span of Saccharomyces cerevisiae cells . During budding yeast cell division, ARS plasmids are retained in the ageing mother cell, such that only 1 out of 10 plasmids enters the rejuvenated bud . Read More
Nature 2010 Jul;466(7305):E1
Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Alliance, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany.
During mitosis in Saccharomyces cerevisiae, senescence factors such as extrachromosomal ribosomal DNA circles (ERCs) are retained in the mother cell and excluded from the bud/daughter cell. Shcheprova et al. proposed a model suggesting segregation of ERCs through their association with nuclear pore complexes (NPCs) and retention of pre-existing NPCs in the mother cell during mitosis. Read More
Nature 2008 Aug 27;454(7205):728-34. Epub 2008 Jul 27.
Institute of Biochemistry, Biology Department, ETH Zurich, Schafmattstrasse 18, 8093 Zurich, Switzerland.
Ageing and the mortality that ensues are sustainable for the species only if age is reset in newborns. In budding yeast, buds are made young whereas ageing factors, such as carbonylated proteins and DNA circles, remain confined to the ageing mother cell. The mechanisms of this confinement and their relevance are poorly understood. Read More
Mol Cell Biol 2005 May;25(10):4283-98
Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712, USA.
The 2 microm circle plasmid in Saccharomyces cerevisiae is a model for a stable, high-copy-number, extrachromosomal "selfish" DNA element. By combining a partitioning system and an amplification system, the plasmid ensures its stable propagation and copy number maintenance, even though it does not provide any selective advantage to its host. Recent evidence suggests that the partitioning system couples plasmid segregation to chromosome segregation. Read More