Screening of differential promoter hypermethylated genes in primary oral squamous cell carcinoma.

Authors:
Mr Mannil Thomas Abraham, MDS, FDSRCS, FFDRCSI, MOMSRCPS
Mr Mannil Thomas Abraham, MDS, FDSRCS, FFDRCSI, MOMSRCPS
Ministry of Health Malaysia
Senior Consultant
Maxillofacial
Klang, Selangor | Malaysia
Associate Professor  Goot Heah Khor, PhD
Associate Professor Goot Heah Khor, PhD
Universiti Teknologi MARA
Dr
Oncology, Molecular Biology
Shah Alam, Selangor | Malaysia

Asian Pac J Cancer Prev 2014 ;15(20):8957-61

Centre of Preclinical Science Studies, Faculty of Medicine, Universiti Teknologi MARA, Selangor, Malaysia E-mail :

Background: Promoter hypermethylation leads to altered gene functions and may result in malignant cellular transformation. Thus, identification of biomarkers for hypermethylated genes could be useful for diagnosis, prognosis, and therapeutic treatment of oral squamous cell carcinoma (OSCC).

Objectives: To screen hypermethylated genes with a microarray approach and to validate selected hypermethylated genes with the methylation-specific polymerase chain reaction (MSPCR).

Materials And Methods: Genome-wide analysis of normal oral mucosa and OSCC tissues was conducted using the Illumina methylation microarray. The specified differential genes were selected and hypermethylation status was further verified with an independent cohort sample of OSCC samples. Candidate genes were screened using microarray assay and run by MSPCR analysis.

Results: TP73, PIK3R5, and CELSR3 demonstrated high percentages of differential hypermethylation status.

Conclusions: Our microarray screening and MSPCR approaches revealed that the signature candidates of differentially hypermethylated genes may possibly become potential biomarkers which would be useful for diagnostic, prognostic and therapeutic targets of OSCC in the near future.

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Source
http://dx.doi.org/10.7314/apjcp.2014.15.20.8957DOI Listing
July 2015
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