Immunochemical authentication of manuka honey using a monoclonal antibody specific to a glycoside of methyl syringate.

Authors:
Yoji Kato
Yoji Kato
University of Hyogo
Japan
Yukako Araki
Yukako Araki
School of Human Science and Environment
Maki Juri
Maki Juri
School of Human Science and Environment
Tuscaloosa | United States
Akari Ishisaka
Akari Ishisaka
Graduate School of Nutrition and Biosciences
Japan
Noritoshi Kitamoto
Noritoshi Kitamoto
School of Human Science and Environment
Japan
Yoko Nitta
Yoko Nitta
University of Hyogo
Japan
Toshio Niwa
Toshio Niwa
Gifu Prefectural Research Institute for Bioengineering
Japan

J Agric Food Chem 2014 Nov 22;62(44):10672-8. Epub 2014 Oct 22.

School of Human Science and Environment and ‡Research Institute for Food and Nutritional Sciences, University of Hyogo , Himeji, Hyogo 670-0092, Japan.

Leptosperin, a novel glycoside of methyl syringate, is exclusively present in manuka honey derived from the Leptospermum species Leptospermum scoparium. Quantification of leptosperin might thus be applicable for authentication of honey. The concentration of leptosperin has high linearity with antibacterial activity. We established a monoclonal antibody to leptosperin and characterized the antibody in detail by a competitive enzyme-linked immunosorbent assay (ELISA), comparing the results with those of the high-performance liquid chromatography (HPLC) method for validation. The antigen in manuka honey was confirmed as leptosperin by HPLC fractionation with quantitation by an ELISA. Leptosperin contents of 50 honey samples were analyzed by an established ELISA, which can handle 20 samples (duplicate) on one 96-well plate. Significant coincidence with the chemical quantitation was observed. Immunochemical quantitation of leptosperin would be an economical and facile method for the possible authentication of manuka honey, allowing many honey samples to be processed and analyzed by an ELISA simultaneously.

Download full-text PDF

Source
http://dx.doi.org/10.1021/jf503464aDOI Listing

Still can't find the full text of the article?

We can help you send a request to the authors directly.
November 2014
116 Reads

Publication Analysis

Top Keywords

manuka honey
16
glycoside methyl
8
methyl syringate
8
honey samples
8
monoclonal antibody
8
authentication manuka
8
leptosperin
7
honey
7
antigen manuka
4
contents honey
4
validation antigen
4
honey confirmed
4
leptosperin contents
4
confirmed leptosperin
4
quantitation elisa
4
elisa leptosperin
4
fractionation quantitation
4
method validation
4
hplc fractionation
4
leptosperin hplc
4

Similar Publications