Enterocytozoon bieneusi in sika deer (Cervus nippon) and red deer (Cervus elaphus): deer specificity and zoonotic potential of ITS genotypes.

Authors:
Wei Zhao
Wei Zhao
School of Public Health
New Haven | United States
Weizhe Zhang
Weizhe Zhang
Harbin Medical University
China
Rongjun Wang
Rongjun Wang
College of Animal Science and Veterinary Medicine
China
Weishi Liu
Weishi Liu
College of Wildlife Resources
Aiqin Liu
Aiqin Liu
Harbin Medical University
Haerbin Shi | China
Dong Yang
Dong Yang
Asan Medical Center
South Korea
Fengkun Yang
Fengkun Yang
Harbin Medical University
China
Dr Md Robiul Karim, PhD
Dr Md Robiul Karim, PhD
Bangabandhu Sheikh Mujibur Rahman Agricultural University
Assistant Professor
Cryptosporidium, Giardia, Microsporidium, Molecular epidemiology, Population genetics
Gazipur | Bangladesh

Parasitol Res 2014 Nov 4;113(11):4243-50. Epub 2014 Sep 4.

Department of Parasitology, Harbin Medical University, Harbin, Heilongjiang, 150081, China.

As the most common cause of the human microsporidiosis, Enterocytozoon bieneusi has been found in a wide variety of animal hosts. Deers are the ruminant mammals living in a variety of biomes, and the distribution of deer species differ by geography. To understand the prevalence of natural infection of E. bieneusi in deer and to assess their epidemiological role in the transmission of microsporidiosis caused by E. bieneusi, 91 fecal specimens were collected from 86 sika deers and five red deers in the northeast of China. By PCR and sequencing of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene of E. bieneusi, an average infection rate of 31.9% (29/91) was observed in deer, with 32.6% (28/86) for sika deer, and 20% (1/5) for red deer. Six ITS genotypes were identified: one known genotype BEB6 (n = 20) and five novel genotypes HLJD-I to HLJD-IV (one each) and HLJD-V (n = 5). A phylogenetic analysis based on a neighbor-joining tree of the ITS gene sequences of E. bieneusi indicated that genotypes HLJD-II and HLJD-III fell into group 1 of zoonotic potential, while the other genotypes (BEB6, HLJD-I, HLJD-IV, HLJD-V) were clustered into so-called bovine-specific group 2. This is the first report of E. bieneusi in deer in China. The observation of genotype BEB6 in humans previously and in deer here and also the findings of the two novel genotypes (HLJD-II to HLJ-III) belonging to potential zoonotic group 1 suggested the possibility of deer in the transmission of E. bieneusi to humans.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00436-014-4100-9DOI Listing

Still can't find the full text of the article?

We can help you send a request to the authors directly.
November 2014
30 Reads
15 Citations
2.330 Impact Factor

Publication Analysis

Top Keywords

deer
11
novel genotypes
8
zoonotic potential
8
hljd-i hljd-iv
8
hljd-iv hljd-v
8
red deer
8
potential genotypes
8
genotype beb6
8
enterocytozoon bieneusi
8
genotypes hljd-ii
8
sika deer
8
deer cervus
8
bieneusi deer
8
bieneusi
7
genotypes
6
transcribed spacer
4
spacer region
4
clustered so-called
4
internal transcribed
4
so-called bovine-specific
4

References

(Supplied by CrossRef)
Article in Am J Trop Med Hyg
FO Akinbo et al.
Am J Trop Med Hyg 2012
Article in Am J Trop Med Hyg
FO Akinbo et al.
Am J Trop Med Hyg 2013
Article in Appl Environ Microbiol
MA Buckholt et al.
Appl Environ Microbiol 2002
Article in J Protozool
V Decraene et al.
J Protozool 1985
Article in J Clin Microbiol
B Dengjel et al.
J Clin Microbiol 2001
Article in J Eukaryot Microbiol
P Deplazes et al.
J Eukaryot Microbiol 1996
Article in Curr Opin Infect Dis
ES Didier et al.
Curr Opin Infect Dis 2006
Article in Parasitol Res
R Fayer et al.
Parasitol Res 2007
Article in PLoS One
AL Galván-Díaz et al.
PLoS One 2014
Article in Interdis Perspect Infect Dis
K Ghosh et al.
Interdis Perspect Infect Dis 2009
Article in Appl Environ Microbiol
Y Guo et al.
Appl Environ Microbiol 2014

Similar Publications