Chemical biology of genomic DNA: minimizing PCR bias.

Chem Commun (Camb) 2014 Oct;50(81):12047-9

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.

The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.

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http://xlink.rsc.org/?DOI=C4CC05107F
Publisher Site
http://dx.doi.org/10.1039/c4cc05107fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759930PMC
October 2014
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