A novel modified electrode as GC/PPy-AuNPs-rGO/L-Cys/Ag@MUA nanostructure configuration for determination of CCP and CRP antibodies in human blood serum samples.

Biosens Bioelectron 2015 Jan 7;63:490-498. Epub 2014 Aug 7.

Department of Chemistry, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.

In this work, silver nanoparticles were synthesized and stabilized with 11-mercaptoundecanoateanions to produce a new Ag@MUA core shell structure, and its utilizing for fabrication of a new sensing film. Gold nanoparticles (AuNPs) were electrochemically produced and simultaneously immobilized into the electropolymerized polypyrrole (PPy) film with the reduced graphene oxide (rGO). The Ag@MUA was then grafted to the surface of GC/PPy-AuNPs-rGO film using L-cysteine (L-Cys) linker agent and trifluoromethanesulfonic anhydride (TF2O), at ambient temperature and under the electrode stirring. The characterization of the sensor was studied by scanning electron microscopy, electrochemical impedance spectroscopy, cyclic and square wave voltammetry techniques. The utility of the modified electrode for clinical diagnosis has been successfully demonstrated by the analysis of human blood serums with a certified CRP and CCP content. Thus, the proposed sensor shows simple preparation, accuracy and precision in the analysis of cytochrome c protein (CCP) and C-reactive protein (CRP (with less side interferences.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2014.07.082DOI Listing
January 2015

Publication Analysis

Top Keywords

modified electrode
8
human blood
8
temperature electrode
4
electrode stirring
4
ambient temperature
4
tf2o ambient
4
trifluoromethanesulfonic anhydride
4
stirring characterization
4
anhydride tf2o
4
characterization sensor
4
microscopy electrochemical
4
electrochemical impedance
4
electron microscopy
4
scanning electron
4
sensor studied
4
studied scanning
4
agent trifluoromethanesulfonic
4
l-cys linker
4
film reduced
4
reduced graphene
4

Similar Publications