FEMS Microbiol Lett 2014 Oct 8;359(1):102-9. Epub 2014 Sep 8.
Institute of Biological Research, Cluj-Napoca, Romania.
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PLoS One 2012 4;7(4):e34983. Epub 2012 Apr 4.
IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.
Cyanobacteria are a group of photosynthetic prokaryotes that have a diverse morphology, minimal nutritional requirements and metabolic plasticity that has made them attractive organisms to use in biotechnological applications. The use of these organisms as cell factories requires the knowledge of their physiology and metabolism at a systems level. For the quantification of gene transcripts real-time quantitative polymerase chain reaction (RT-qPCR) is the standard technique. Read More
Antonie Van Leeuwenhoek 2015 Sep 7;108(3):685-93. Epub 2015 Jul 7.
Institute of Health Sciences, Federal University of Bahia, Salvador, BA, Brazil.
The appropriate choice of reference genes is essential for accurate normalization of gene expression data obtained by the method of reverse transcription quantitative real-time PCR (RT-qPCR). In 2009, a guideline called the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) highlighted the importance of the selection and validation of more than one suitable reference gene for obtaining reliable RT-qPCR results. Herein, we searched the recent literature in order to identify the bacterial reference genes that have been most commonly validated in gene expression studies by RT-qPCR (in the first 5 years following publication of the MIQE guidelines). Read More
Anaerobe 2014 Jun 19;27:34-9. Epub 2014 Mar 19.
Department of Agricultural Sciences, University of Bologna, viale Fanin 42, 40127 Bologna, Italy.
Normalization is an essential prerequisite for producing accurate real-time PCR expression analyses. The objective of this study is the selection of a set of optimal reference genes in Bifidobacterium adolescentis gene expression studies under bile exposure. B adolescentis is a particularly abundant species in the human adults gut microbiota, exerting relevant probiotic activities. Read More
PLoS One 2013 10;8(12):e82927. Epub 2013 Dec 10.
State Key Laboratory of Tree Genetics and Breeding, Beijing, China ; Key Laboratory of Tree Genomics, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, Zhejiang, China.
Real-time Quantitative PCR (RT-qPCR) has become an effective method for accurate analysis of gene expression in several biological systems as well as under different experimental conditions. Although with high sensitivity, specificity and broad dynamic range, this method requires suitable reference genes for transcript normalization in order to guarantee reproducible and meaningful results. In the present study, we evaluated five traditional housekeeping genes and five novel reference genes in Hyperaccumulating ecotype of Sedum alfredii, a well known hyperaccumulator for heavy metals phytoremediation, under Cd, Pb, Zn and Cu stresses of seven different durations. Read More