Sequential glycan profiling at single cell level with the microfluidic lab-in-a-trench platform: a new era in experimental cell biology.

Dermot Walls
Dermot Walls
Dublin City University
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Fraunhofer Project Centre at Dublin City University
Professor (Full)
microfluidics, Lab-on-a-Chip, hydrodynanmics, business development, project management, organisational leadership
Glasnevin, Dublin 9 | Ireland

Lab Chip 2014 Sep 31;14(18):3629-39. Epub 2014 Jul 31.

School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.

It is now widely recognised that the earliest changes that occur on a cell when it is stressed or becoming diseased are alterations in its surface glycosylation. Current state-of-the-art technologies in glycoanalysis include mass spectrometry, protein microarray formats, techniques in cytometry and more recently, glyco-quantitative polymerase chain reaction (Glyco-qPCR). Techniques for the glycoprofiling of the surfaces of single cells are either limited to the analysis of large cell populations or are unable to handle multiple and/or sequential probing. Here, we report a novel approach of single live cell glycoprofiling enabled by the microfluidic "Lab-in-a-Trench" (LiaT) platform for performing capture and retention of cells, along with shear-free reagent loading and washing. The significant technical improvement on state-of-the-art is the demonstration of consecutive, spatio-temporally profiling of glycans on a single cell by sequential elution of the previous lectin probe using their corresponding free sugar. We have qualitatively analysed glycan density on the surface of individual cells. This has allowed us to qualitatively co-localise the observed glycans. This approach enables exhaustive glycoprofiling and glycan mapping on the surface of individual live cells with multiple lectins. The possibility of sequentially profiling glycans on cells will be a powerful new tool to add to current glycoanalytical techniques. The LiaT platform will enable cell biologists to perform many high sensitivity assays and also will also make a significant impact on biomarker research.

Download full-text PDF

Source Listing

Still can't find the full text of the article?

Sign up to send a request to the authors directly.
September 2014
34 Reads
1 PubMed Central Citation(source)

Publication Analysis

Top Keywords

single cell
surface individual
liat platform
profiling glycans
allowed qualitatively
multiple and/or
handle multiple
qualitatively co-localise
co-localise observed
unable handle
approach single
and/or sequential
probing report
novel approach
individual cells
populations unable
cells allowed
sequential probing


(Supplied by CrossRef)
Article in Cell
Altschuler et al.
Cell 2010
Article in Annu. Rev. Pathol.: Mech. Dis.
Almendro et al.
Annu. Rev. Pathol.: Mech. Dis. 2013
Article in Nat. Rev. Drug Discovery
Dube et al.
Nat. Rev. Drug Discovery 2005

Varki et al.
Article in Cell
Ohtsubo et al.
Cell 2006
Article in Acta Histochem.
Ghazarian et al.
Acta Histochem. 2011

Article in Annu. Rev. Anal. Chem.
Rakus et al.
Annu. Rev. Anal. Chem. 2011
Article in Org. Biomol. Chem.
Ambrosi et al.
Org. Biomol. Chem. 2005
Article in Electrophoresis
Hirabayashi et al.
Electrophoresis 2011
Article in Nat. Methods
Kuno et al.
Nat. Methods 2005

Similar Publications

The Effects of Omega-3 and Branched-Chain Amino Acids Supplementation on Serum Apoptosis Markers Following Acute Resistance Exercise in Old Men.

J Aging Phys Act 2018 Aug 17:1-23. Epub 2018 Aug 17.

3 Department of Physical Education and Sports Science, Marivan Branch, Islamic Azad University, Marivan, Iran.

The potential benefits of Omega-3 fatty acids and branched-chain amino acids (BCAAs) supplements on exercise-induced apoptosis are not clear. In a crossover randomized study, 11 men (age 62.8 ± 2. Read More

View Article
August 2018
20 Reads
1.41 Impact Factor

A clinical-pathogenetic approach on associated anomalies and chromosomal defects supports novel candidate critical regions and genes for gastroschisis.

Pediatr Surg Int 2018 Sep 9;34(9):931-943. Epub 2018 Aug 9.

Departamento de Genética, Facultad de Medicina y Hospital Universitario José Eleuterio González, Universidad Autónoma de Nuevo León, Ave. Madero y Gonzalitos S/N Col. Mitras Centro, CP 64460, Monterrey, Nuevo León, México.

Background: Gastroschisis has been assumed to have a low rate of syndromic and primary malformations. We aimed to systematically review and explore the frequency and type of malformations/chromosomal syndromes and to identify significant biological/genetic roles in gastroschisis.

Methods: Population-based, gastroschisis-associated anomalies/chromosomal defects published 1950-2018 (PubMed/MEDLINE) were independently searched by two reviewers. Read More

View Article
September 2018
23 Reads
1.06 Impact Factor

N-methyl-D-aspartate receptors involved in morphine-induced hyperalgesia in sensitized mice.

Eur J Pharmacol 2014 Aug 16;737:85-90. Epub 2014 May 16.

Department of Statistics, Faculty of Science, University of Kurdistan, Sanandaj, Iran.

The aim of this study was to investigate role of the N-Methyl-D-Aspartate (NMDA) receptors in the decrease of morphine analgesia in mice after nociceptive sensitization. We used a hot plate test to assess effects of morphine on pain behavior in male NMRI mice. All drugs were administered through an intraperitoneal route. Read More

View Article
August 2014
16 Reads
4 PubMed Central Citations(source)
2.53 Impact Factor

Agricultural work related injuries among the farmers of West Bengal, India.

Banibrata Das

Int J Inj Contr Saf Promot 2014 15;21(3):205-15. Epub 2013 May 15.

a Department of Physiology, South Calcutta Girls' College , University of Calcutta , Kolkata 700025 , India.

Agriculture is one of the most hazardous industries with high rates of accidental death, injury and occurrences of work related illnesses. The main objective of the study is to assess types, categories and number of incident rate of the accidents along with the causes of accidents as well as with the affected parts of the different parts of the body among the agricultural workers. The number of injuries that occurred during 2006-2010 for male and female farmers was 214 and 109, respectively, in West Bengal. Read More

View Article
May 2015
55 Reads
3 PubMed Central Citations(source)

Glandular trichomes of Ceratotheca triloba (Pedaliaceae): morphology, histochemistry and ultrastructure.

Planta 2012 Oct 14;236(4):1215-26. Epub 2012 Jun 14.

School of Life Sciences, University of KwaZulu-Natal, P/Bag X54001, Durban 4000, South Africa.

This study was initiated to characterize the distribution, morphology, secretion mode, histochemistry and ultrastructure of the glandular trichomes of Ceratotheca triloba using light and electron microscopy. Its leaves bear two morphologically distinct glandular trichomes. The first type has long trichome with 8-12 basal cells of pedestal, 3-14 stalk cells, a neck cell and a head of four cells in one layer. Read More

View Article
October 2012
20 Reads
1 PubMed Central Citation(source)
3.26 Impact Factor