Analyzing the movement of the Nauplius 'Artemia salina' by optical tracking of plasmonic nanoparticles.

Authors:
Silke R Kirchner
Silke R Kirchner
Ludwig-Maximilians-Universität
Bad Reichenhall | Germany
Michael Fedoruk
Michael Fedoruk
Ludwig-Maximilians-Universität München
Germany
Jochen Feldmann
Jochen Feldmann
Ludwig-Maximilians-Universität München

J Vis Exp 2014 Jul 15(89). Epub 2014 Jul 15.

Photonics and Optoelectronics Group, Ludwig-Maximilians-Universität.

We demonstrate how optical tweezers may provide a sensitive tool to analyze the fluidic vibrations generated by the movement of small aquatic organisms. A single gold nanoparticle held by an optical tweezer is used as a sensor to quantify the rhythmic motion of a Nauplius larva (Artemia salina) in a water sample. This is achieved by monitoring the time dependent displacement of the trapped nanoparticle as a consequence of the Nauplius activity. A Fourier analysis of the nanoparticle's position then yields a frequency spectrum that is characteristic to the motion of the observed species. This experiment demonstrates the capability of this method to measure and characterize the activity of small aquatic larvae without the requirement to observe them directly and to gain information about the position of the larvae with respect to the trapped particle. Overall, this approach could give an insight on the vitality of certain species found in an aquatic ecosystem and could expand the range of conventional methods for analyzing water samples.

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http://dx.doi.org/10.3791/51502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4217367PMC
July 2014
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