Enzymatic synthesis and purification of a defined RIG-I ligand.

Methods Mol Biol 2014 ;1169:15-25

Institute for Clinical Chemistry and Clinical Pharmacology, University Hospital, University of Bonn, Sigmund-Freud-Strasse 25, 53127, Bonn, Germany.

Receptor-based nucleic acid sensing constitutes one of the most fundamental mechanisms of our innate immune system to sense viral infection. RIG-I is a cytosolic RNA helicase that senses the presence of 5' triphosphate RNA species, a common feature of many negative strand RNA viruses. We here describe a protocol to enzymatically synthesize and to purify a defined RIG-I ligand that can be used to study RIG-I activation in vitro and in vivo.

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http://dx.doi.org/10.1007/978-1-4939-0882-0_2DOI Listing
February 2015
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