A monoterpene, unique component of thyme honeys, induces apoptosis in prostate cancer cells via inhibition of NF-κB activity and IL-6 secretion.

Authors:
Eva Kassi
Eva Kassi
Medical School
Boston | United States
Ioanna Chinou
Ioanna Chinou
University of Athens
Athens | Greece
Eliana Spilioti
Eliana Spilioti
University of Athens
Greece
Anna Tsiapara
Anna Tsiapara
Medical School
Greece
Konstantia Graikou
Konstantia Graikou
University of Athens
Greece
Sofia Karabournioti
Sofia Karabournioti
Medical School
Greece
Menelaos Manoussakis
Menelaos Manoussakis
School of Medicine
Salt Lake City | United States
Paraskevi Moutsatsou
Paraskevi Moutsatsou
Medical School
Boston | United States

Phytomedicine 2014 Sep 2;21(11):1483-9. Epub 2014 Jun 2.

Department of Biological Chemistry, Medical School, University of Athens, 75 Mikras Asias Str., 11527 Goudi, Athens, Greece. Electronic address:

We have previously demonstrated that Greek thyme honey inhibits significantly the cell viability of human prostate cancer cells. Herein, 15 thyme honey samples from several regions of Greece were submitted to phytochemical analysis for the isolation, identification and determination (through modern spectral means) of the unique thyme honey monoterpene, the compound trihydroxy ketone E-4-(1,2,4-trihydroxy-2,6,6-trimethylcyclohexyl)-but-3-en-2-one. We investigated the anti-growth and apoptotic effects of the trihydroxy ketone on PC-3 human androgen independent prostate cancer cells using MTT assay and Annexin V-FITC respectively. The molecular pathways involved to such effects were further examined by evaluating its ability to inhibit (a) the NF-κB phosphorylation (S536), (b) JNK and Akt phosphorylation (Thr183/Tyr185 and S473 respectively) and (c) IL-6 production, using ELISA method. The anti-microbial effects of the trihydroxy ketone against a panel of nine pathogenic bacteria and three fungi were also assessed. The trihydroxy ketone exerted significant apoptotic activity in PC-3 prostate cancer cells at 100 μM, while it inhibited NF-κB phosphorylation and IL-6 secretion at a concentration range 10(-6)-10(-4)M. Akt and JNK signaling were not found to participate in this process. The trihydroxy ketone exerted significant anti-microbial profile against many human pathogenic bacteria and fungi (MIC values ranged from 0.04 to 0.57 mg/ml). Conclusively, the Greek thyme honey-derived monoterpene exerted significant apoptotic activity in PC-3 cells, mediated, at least in part, through reduction of NF-κB activity and IL-6 secretion and may play a key role in the anti-growth effect of thyme honey on prostate cancer cells.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S09447113140021
Publisher Site
http://dx.doi.org/10.1016/j.phymed.2014.04.032DOI Listing

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September 2014
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