Detection of Plasmodium vivax and Plasmodium falciparum DNA in human saliva and urine: loop-mediated isothermal amplification for malaria diagnosis.

Authors:
Hormozd Oormazdi
Hormozd Oormazdi
School of Medicine
Lame Akhlaghi
Lame Akhlaghi
School of Medicine
Ahmad Reza Meamar
Ahmad Reza Meamar
School of Medicine
Mehdi Nateghpour
Mehdi Nateghpour
School of Public Health
Iran
Leila Farivar
Leila Farivar
School of Public Health
Iran
Elham Razmjou
Elham Razmjou
School of Medicine
Iran

Acta Trop 2014 Aug 8;136:44-9. Epub 2014 Apr 8.

Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address:

This study investigated loop-mediated isothermal amplification (LAMP) detection of Plasmodium falciparum and Plasmodium vivax in urine and saliva of malaria patients. From May to November 2011, 108 febrile patients referred to health centers in Sistan and Baluchestan Province of south-eastern Iran participated in the study. Saliva, urine, and blood samples were analyzed with nested PCR and LAMP targeting the species-specific nucleotide sequence of small subunit ribosomal RNA gene (18S rRNA) of P. falciparum and P. vivax and evaluated for diagnostic accuracy by comparison to blood nested PCR assay. When nested PCR of blood is used as standard, microscopy and nested PCR of saliva and urine samples showed sensitivity of 97.2%, 89.4% and 71% and specificity of 100%, 97.3% and 100%, respectively. LAMP sensitivity of blood, saliva, and urine was 95.8%, 47% and 29%, respectively, whereas LAMP specificity of these samples was 100%. Microscopy and nested PCR of saliva and LAMP of blood were comparable to nested PCR of blood (к=0.95, 0.83, and 0.94, respectively), but agreement for nested PCR of urine was moderate (к=0.64) and poor to fair for saliva LAMP and urine LAMP (к=0.38 and 0.23, respectively). LAMP assay showed low sensitivity for detection of Plasmodium DNA in human saliva and urine compared to results with blood and to nested PCR of blood, saliva, and urine. However, considering the advantages of LAMP technology and of saliva and urine sampling, further research into the method is worthwhile. LAMP protocol and precise preparation protocols need to be defined and optimized for template DNA of saliva and urine.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actatropica.2014.03.029DOI Listing

Still can't find the full text of the article?

Sign up to send a request to the authors directly.
August 2014
7 Reads

Publication Analysis

Top Keywords

saliva urine
32
nested pcr
32
pcr blood
12
detection plasmodium
12
urine
11
saliva
11
lamp
10
loop-mediated isothermal
8
plasmodium vivax
8
blood nested
8
isothermal amplification
8
blood saliva
8
pcr saliva
8
microscopy nested
8
nested
8
saliva lamp
8
pcr
8
dna human
8
blood
8
plasmodium falciparum
8

Similar Publications

Diagnosis, Evaluation, and Management of High Blood Pressure in Children and Adolescents.

Pediatrics 2018 Sep 20;142(3). Epub 2018 Aug 20.

Preventive Cardiology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.

Systemic hypertension is a major cause of morbidity and mortality in adulthood. High blood pressure (HBP) and repeated measures of HBP, hypertension (HTN), begin in youth. Knowledge of how best to diagnose, manage, and treat systemic HTN in children and adolescents is important for primary and subspecialty care providers. Read More

View Article
September 2018
25 Reads
5.47 Impact Factor

Defines a Glycolytic Subpopulation and White Adipocyte Heterogeneity.

Diabetes 2017 11 28;66(11):2822-2829. Epub 2017 Aug 28.

Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA

is a member of the T-box gene family of mesodermal developmental genes. We have recently shown that Tbx15 plays a critical role in the formation and metabolic programming of glycolytic myofibers in skeletal muscle. is also differentially expressed among white adipose tissue (WAT) in different body depots. Read More

View Article
November 2017
17 Reads
2 PubMed Central Citations(source)
8.10 Impact Factor

Pathology-Driven Comprehensive Proteomic Profiling of the Prostate Cancer Tumor Microenvironment.

Mol Cancer Res 2017 03 5;15(3):281-293. Epub 2017 Jan 5.

UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland.

Prostate cancer is the second most common cancer in men worldwide. Gleason grading is an important predictor of prostate cancer outcomes and is influential in determining patient treatment options. Clinical decisions based on a Gleason score of 7 are difficult as the prognosis for individuals diagnosed with Gleason 4+3 cancer is much worse than for those diagnosed with Gleason 3+4 cancer. Read More

View Article
March 2017
19 Reads
3 PubMed Central Citations(source)
4.38 Impact Factor

A CatalyCEST MRI Contrast Agent that Can Simultaneously Detect Two Enzyme Activities.

Chembiochem 2016 Mar 3;17(5):383-7. Epub 2016 Feb 3.

Department of Medical Imaging, University of Arizona, 1515 N. Campbell Ave., Tucson, AZ, 85724, USA.

The simultaneous detection of multiple enzyme activities can improve the specificity of disease diagnoses. We therefore synthesized and characterized a diamagnetic chemical exchange saturation transfer (CEST) MRI contrast agent that can simultaneously detect two enzyme activities. Sulfatase and esterase enzymes cleave the ligands of the CEST agent, releasing salicylic acid that can be detected with CEST MRI. Read More

View Article
March 2016
19 Reads

Nonlinear enhancement of oxygen evolution in thylakoid membranes: modeling the effect of light intensity and beta-cyclodextrin concentration.

J Phys Chem B 2005 Aug;109(30):14707-14

Université du Québec à Trois-Rivières, Département de Chimie-Biologie, Section de Chimie et Biochimie, Trois-Rivières, Québec G9A 5H7, Canada.

Electron transport through photosystem II, measured as oxygen evolution (OE), was investigated in isolated thylakoid membranes treated with beta-cyclodextrin (beta-CD, a cyclic oligosaccharide constituted of seven alpha-d-glucose residues linked by alpha-1,4 glycosidic bonds) and irradiated with white light of variable intensity. First, we found that the light-response curves of oxygen evolution are well fitted with a hyperbolic function, the shape of which is not affected by the beta-CD concentration. Second, we showed that under conditions of irradiation with white light of saturating intensity ( approximately 5000 mumol of photons/m(2). Read More

View Article
August 2005
4 Reads