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Modulation effect of low-frequency electric and magnetic fields on CO2 production and rates of acetate and pyruvate formation in Saccharomyces cerevisiae cell culture.
Electromagn Biol Med 2015 Mar 2;34(1):93-104. Epub 2014 Apr 2.
Department of Physics, UPR , Rio Piedras, San Juan , Puerto Rico and.
We studied action of one-dimensional, two-dimensional and three-dimensional low-frequency oscillating electric and magnetic fields on sugar metabolism in Saccharomyces cerevisiae cell culture. S. cerevisiae cells were grown on a minimal medium containing glucose (10%) as a carbon source and salts (0.3-0.5%) that supplied nitrogen, phosphorus and trace metals. We found that appropriate three-dimensional field patterns can either accelerate or inhibit sugar metabolism in yeast cells, as compared to control experiments. We also studied aerobic sugar metabolism, with similar results. Sugar metabolism was monitored by formation of pyruvate, acetate and CO2. We found that for the P1 parameter set the cell metabolism accelerates as evaluated by all of the monitored chemical products, and the cell density growth rate also accelerates, with opposite effects observed for the P2 parameter set. These parameter sets are introduced using D, ω, φ, B, ω', and φ' - vectors defining amplitudes, frequencies and phases of periodic electric and magnetic fields, respectively. Thus, the P1 parameter set: D = (2.6, 3.1, 2.2) V/cm; ω = (0.8, 1.6, 0.2) kHz; φ = (1.31, 0.9, 1.0) rad; B = (3.1, 7.2, 7.2) × 10(-4) T; ω' = (2.1, 1.3, 3.1) kHz; φ' = (0.4, 2.1, 2.8) rad; and the P2 parameter set: D = (4.3, 1.6, 3.8) V/cm; ω = (3.3, 1.8, 2.8) kHz; φ = (0.86, 1.1, 0.4) rad; B = (5.4, 1.3, 1.3) × 10(-4) T; ω' = (1.3, 1.7, 0.9) kHz; φ' = (2.6, 1.7, 1.7) rad. The effects obtained for the less complex field combinations that used one-dimensional or two-dimensional configurations, or omitted either the electric or the magnetic contribution, were significantly weaker than those obtained for the complete P1 and P2 parameter sets.
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