Modulation effect of low-frequency electric and magnetic fields on CO2 production and rates of acetate and pyruvate formation in Saccharomyces cerevisiae cell culture.

Vladimir I Makarov
Vladimir I Makarov
University of Puerto Rico
San Juan | Puerto Rico
Dr. Igor Khmelinskii, PhD, Prof. Agregado
Dr. Igor Khmelinskii, PhD, Prof. Agregado
University of the Algarve
PhD, Prof. Agregado
physcal chemistry; spectroscopy; climate science
Faro, Algarve | Portugal

Electromagn Biol Med 2015 Mar 2;34(1):93-104. Epub 2014 Apr 2.

Department of Physics, UPR , Rio Piedras, San Juan , Puerto Rico and.

We studied action of one-dimensional, two-dimensional and three-dimensional low-frequency oscillating electric and magnetic fields on sugar metabolism in Saccharomyces cerevisiae cell culture. S. cerevisiae cells were grown on a minimal medium containing glucose (10%) as a carbon source and salts (0.3-0.5%) that supplied nitrogen, phosphorus and trace metals. We found that appropriate three-dimensional field patterns can either accelerate or inhibit sugar metabolism in yeast cells, as compared to control experiments. We also studied aerobic sugar metabolism, with similar results. Sugar metabolism was monitored by formation of pyruvate, acetate and CO2. We found that for the P1 parameter set the cell metabolism accelerates as evaluated by all of the monitored chemical products, and the cell density growth rate also accelerates, with opposite effects observed for the P2 parameter set. These parameter sets are introduced using D, ω, φ, B, ω', and φ' - vectors defining amplitudes, frequencies and phases of periodic electric and magnetic fields, respectively. Thus, the P1 parameter set: D = (2.6, 3.1, 2.2) V/cm; ω = (0.8, 1.6, 0.2) kHz; φ = (1.31, 0.9, 1.0) rad; B = (3.1, 7.2, 7.2) × 10(-4) T; ω' = (2.1, 1.3, 3.1) kHz; φ' = (0.4, 2.1, 2.8) rad; and the P2 parameter set: D = (4.3, 1.6, 3.8) V/cm; ω = (3.3, 1.8, 2.8) kHz; φ = (0.86, 1.1, 0.4) rad; B = (5.4, 1.3, 1.3) × 10(-4) T; ω' = (1.3, 1.7, 0.9) kHz; φ' = (2.6, 1.7, 1.7) rad. The effects obtained for the less complex field combinations that used one-dimensional or two-dimensional configurations, or omitted either the electric or the magnetic contribution, were significantly weaker than those obtained for the complete P1 and P2 parameter sets.

Download full-text PDF

Source Listing
March 2015
5 Reads

Publication Analysis

Top Keywords

electric magnetic
parameter set
sugar metabolism
magnetic fields
cell culture
cerevisiae cell
one-dimensional two-dimensional
parameter sets
saccharomyces cerevisiae
density growth
growth rate
rate accelerates
accelerates opposite
cell density
products cell
evaluated monitored
monitored chemical
chemical products

Similar Publications

FTIR and UV spectroscopy in real-time monitoring of S. cerevisiae cell culture.

Electromagn Biol Med 2011 Dec;30(4):181-97

Department of Chemistry, University of Puerto Rico, San Juan, Puerto Rico.

A combination of FTIR and UV spectroscopy is proposed as a novel technique for integrated real-time monitoring of metabolic activity and growth rates of cell cultures, required for systematic studies of cellular low-frequency (LF) electric and magnetic field (EMF) effects. As an example, we investigated simultaneous influence of periodic LF 3D EMFs on a culture of Saccharomyces cerevisiae (baker's yeast) cells. Amplitudes, frequencies and phases of the field components were the variable parameters. Read More

View Article
December 2011

Initiation of yeast sporulation of partial carbon, nitrogen, or phosphate deprivation.

J Bacteriol 1982 Mar;149(3):840-51

In this paper we show that partial deprivation of a carbon source, a nitrogen source, or phosphate in the presence of all other nutrients needed for growth initiates meiosis and sporulation of Saccharomyces cerevisiae homothallic strain Y55. For carbon deprivation experiments, cells were grown in synthetic medium (pH 5.5) containing an excess of one carbon source and then transferred to the same medium containing different concentrations of the same carbon source. Read More

View Article
March 1982

Steady-state and transient-state analysis of growth and metabolite production in a Saccharomyces cerevisiae strain with reduced pyruvate-decarboxylase activity.

Biotechnol Bioeng 1999 ;66(1):42-50

Kluyver Institute of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.

Pyruvate decarboxylase is a key enzyme in the production of low-molecular-weight byproducts (ethanol, acetate) in biomass-directed applications of Saccharomyces cerevisiae. To investigate whether decreased expression levels of pyruvate decarboxylase can reduce byproduct formation, the PDC2 gene, which encodes a positive regulator of pyruvate-decarboxylase synthesis, was inactivated in the prototrophic strain S. cerevisiae CEN. Read More

View Article
January 2000

Autonomous metabolic oscillation in continuous culture of Saccharomyces cerevisiae grown on ethanol.

FEMS Microbiol Lett 1996 Sep;142(2-3):253-8

Biochemical Engineering Laboratory, National Institute of Bioscience and Human-Technology, Ibaraki, Japan.

When the yeast Saccharomyces cerevisiae was grown under aerobic continuous culture conditions with a medium containing ethanol as carbon source, an autonomous sustained metabolic oscillation appeared. This oscillation was observed in rates and concentrations of various parameters such as, ethanol, oxygen uptake rate, carbon dioxide evolution rate, NaOH addition rate for pH control, acetate, and intracellular pH. No changes were observed in concentrations of stock carbohydrates. Read More

View Article
September 1996