Electromagn Biol Med 2015 Mar 2;34(1):93-104. Epub 2014 Apr 2.
Department of Physics, UPR , Rio Piedras, San Juan , Puerto Rico and.
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Electromagn Biol Med 2011 Dec;30(4):181-97
Department of Chemistry, University of Puerto Rico, San Juan, Puerto Rico.
A combination of FTIR and UV spectroscopy is proposed as a novel technique for integrated real-time monitoring of metabolic activity and growth rates of cell cultures, required for systematic studies of cellular low-frequency (LF) electric and magnetic field (EMF) effects. As an example, we investigated simultaneous influence of periodic LF 3D EMFs on a culture of Saccharomyces cerevisiae (baker's yeast) cells. Amplitudes, frequencies and phases of the field components were the variable parameters. Read More
J Bacteriol 1982 Mar;149(3):840-51
In this paper we show that partial deprivation of a carbon source, a nitrogen source, or phosphate in the presence of all other nutrients needed for growth initiates meiosis and sporulation of Saccharomyces cerevisiae homothallic strain Y55. For carbon deprivation experiments, cells were grown in synthetic medium (pH 5.5) containing an excess of one carbon source and then transferred to the same medium containing different concentrations of the same carbon source. Read More
Biotechnol Bioeng 1999 ;66(1):42-50
Kluyver Institute of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Pyruvate decarboxylase is a key enzyme in the production of low-molecular-weight byproducts (ethanol, acetate) in biomass-directed applications of Saccharomyces cerevisiae. To investigate whether decreased expression levels of pyruvate decarboxylase can reduce byproduct formation, the PDC2 gene, which encodes a positive regulator of pyruvate-decarboxylase synthesis, was inactivated in the prototrophic strain S. cerevisiae CEN. Read More
FEMS Microbiol Lett 1996 Sep;142(2-3):253-8
Biochemical Engineering Laboratory, National Institute of Bioscience and Human-Technology, Ibaraki, Japan.
When the yeast Saccharomyces cerevisiae was grown under aerobic continuous culture conditions with a medium containing ethanol as carbon source, an autonomous sustained metabolic oscillation appeared. This oscillation was observed in rates and concentrations of various parameters such as, ethanol, oxygen uptake rate, carbon dioxide evolution rate, NaOH addition rate for pH control, acetate, and intracellular pH. No changes were observed in concentrations of stock carbohydrates. Read More