Get 20% Off Journals at LWW.com

A smart platform for hyperthermia application in cancer treatment: cobalt-doped ferrite nanoparticles mineralized in human ferritin cages.

ACS Nano 2014 May 8;8(5):4705-19. Epub 2014 Apr 8.

INSTM and Dipartimento di Chimica "U. Schiff", Università degli Studi di Firenze , via della Lastruccia 3, I-50019 Sesto Fiorentino, Italy.

Magnetic nanoparticles, MNPs, mineralized within a human ferritin protein cage, HFt, can represent an appealing platform to realize smart therapeutic agents for cancer treatment by drug delivery and magnetic fluid hyperthermia, MFH. However, the constraint imposed by the inner diameter of the protein shell (ca. 8 nm) prevents its use as heat mediator in MFH when the MNPs comprise pure iron oxide. In this contribution, we demonstrate how this limitation can be overcome through the controlled doping of the core with small amount of Co(II). Highly monodisperse doped iron oxide NPs with average size of 7 nm are mineralized inside a genetically modified variant of HFt, carrying several copies of α-melanocyte-stimulating hormone peptide, which has already been demonstrated to have excellent targeting properties toward melanoma cells. HFt is also conjugated to poly(ethylene glycol) molecules to increase its in vivo stability. The investigation of hyperthermic properties of HFt-NPs shows that a Co doping of 5% is enough to strongly enhance the magnetic anisotropy and thus the hyperthermic efficiency with respect to the undoped sample. In vitro tests performed on B16 melanoma cell line demonstrate a strong reduction of the cell viability after treatment with Co doped HFt-NPs and exposure to the alternating magnetic field. Clear indications of an advanced stage of apoptotic process is also observed from immunocytochemistry analysis. The obtained data suggest this system represents a promising candidate for the development of a protein-based theranostic nanoplatform.

Download full-text PDF

Source
http://dx.doi.org/10.1021/nn500454nDOI Listing
May 2014
Get 20% Off Journals at LWW.com

Similar Publications

Regeneration of pulp-dentin complex using human stem cells of the apical papilla: in vivo interaction with two bioactive materials.

Clin Oral Investig 2021 Feb 25. Epub 2021 Feb 25.

Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.

Objectives: To compare the regenerative properties of human stem cells of the apical papilla (SCAPs) embedded in a platelet-rich plasma (PRP) scaffold, when implanted in vivo using an organotypic model composed of human root segments, with or without the presence of the bioactive cements - ProRoot MTA or Biodentine.

Material And Methods: SCAPs were isolated from third molars with incomplete rhizogenesis and expanded and characterized in vitro using stem cell and surface markers. The pluripotency of these cells was also assessed using adipogenic, chondrogenic, and osteogenic differentiation protocols. Read More

View Article and Full-Text PDF
February 2021

Multipronged dental analyses reveal dietary differences in last foragers and first farmers at Grotta Continenza, central Italy (15,500-7000 BP).

Sci Rep 2021 Feb 19;11(1):4261. Epub 2021 Feb 19.

Department of Maxillo-Facial Sciences, DANTE - Diet and Ancient Technology Laboratory, Sapienza University of Rome, Rome, Italy.

This paper provides results from a suite of analyses made on human dental material from the Late Palaeolithic to Neolithic strata of the cave site of Grotta Continenza situated in the Fucino Basin of the Abruzzo region of central Italy. The available human remains from this site provide a unique possibility to study ways in which forager versus farmer lifeways affected human odonto-skeletal remains. The main aim of our study is to understand palaeodietary patterns and their changes over time as reflected in teeth. Read More

View Article and Full-Text PDF
February 2021

rAAV-Mediated Human FGF-2 Gene Therapy Enhances Osteochondral Repair in a Clinically Relevant Large Animal Model Over Time In Vivo.

Am J Sports Med 2021 Feb 19:363546521988941. Epub 2021 Feb 19.

Center of Experimental Orthopaedics, Saarland University Medical Center and Saarland University, Homburg/Saar, Germany.

Background: Osteochondral defects, if left untreated, do not heal and can potentially progress toward osteoarthritis. Direct gene transfer of basic fibroblast growth factor 2 (FGF-2) with the clinically adapted recombinant adeno-associated viral (rAAV) vectors is a powerful tool to durably activate osteochondral repair processes.

Purpose: To examine the ability of an rAAV-FGF-2 construct to target the healing processes of focal osteochondral injury over time in a large translational model in vivo versus a control gene transfer condition. Read More

View Article and Full-Text PDF
February 2021

[The potential of using the underground mineral waters of the Moscow region in the organization of medical rehabilitation and medical tourism].

Vopr Kurortol Fizioter Lech Fiz Kult 2021 ;98(1):53-57

Moscow Research and Practical Centre for of Medical Rehabilitation, Restorative and Sports Medicine, Moscow, Russia.

Moscow in modern borders is the largest metropolitan area in the country with a fairly high and unevenly distributed population density. This predetermines the need to address environmental regulation of urbanization in order to create the most comfortable living conditions for the population. Health care and the maintenance of health of all age groups are the leading role in the list of the most important tasks to ensure the social well-being of citizens. Read More

View Article and Full-Text PDF
February 2021

MiR-143-3p Inhibits Osteogenic Differentiation of Human Periodontal Ligament Cells by Targeting KLF5 and Inactivating the Wnt/β-Catenin Pathway.

Front Physiol 2020 2;11:606967. Epub 2021 Feb 2.

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, China.

Human periodontal ligament cells (hPDLCs) play a vital role in cell regeneration and tissue repair with multi-directional differentiation potential. microRNAs (miRs) are implicated in the osteogenesis of hPDLCs. This study explored the mechanism of miR-143-3p in osteogenesis of hPDLCs. Read More

View Article and Full-Text PDF
February 2021