Expression of the metastasis suppressor BRMS1 in uveal melanoma.

Ecancermedicalscience 2014 11;8:410. Epub 2014 Mar 11.

Department of Ophthalmology and Pathology, The Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, Quebec H3A 2B4, Canada.

Aims: To determine the expression of breast metastasis suppressor 1 (BRMS1) in human uveal melanoma (UM) tissues and cell lines. In addition, we intend to establish a possible association between BRMS1 expression and the presence of metastatic disease.

Methods: Thirty-one formalin-fixed paraffin-embedded tissues from enucleated eyes of patients with UM were immunostained. Clinical-pathological data were obtained, including age, tumour location, largest dimension, cell type, and occurrence of metastasis. The expression of BRMS1 mRNA in four human UM cell lines was determined by real-time reverse transcriptase polymerase chain reaction, and protein expression was assessed by immunocytochemistry and western blot. The association between BRMS1 immunostaining and location, largest tumour dimension, and tumour cell type was determined using the correlation coefficient test. The association between BRMS1 immunostaining and the incidence of metastasis was assessed using Kaplan-Meier analysis.

Results: Of the 31 cases of UM, 24 (77.42%) stained positive and seven (22.58%) negative for BRMS1. From the positively stained tumours, 21 (87.50%) showed cytoplasmatic staining. Macrophages were usually positive when present in the tumour and staining intensity was generally higher than in UM cells. BRMS1 mRNA was present in all four human UM cell lines, as well as cytoplasmatic immunoexpression of BRMS1. Immunoblotting showed variable BRMS1 protein levels between the different cell lines. No statistically significant correlation was found between BRMS1 protein expression and survival (P = 0.69), tumour cell type (P = 0.68), largest tumour dimension (P = 0.75), and tumour location (P = 0.11).

Conclusions: BRMS1 is expressed in UM both at the mRNA and protein level; however, neither was associated with any of the prognosticor outcome parameters that we tested.

Download full-text PDF

Source
http://dx.doi.org/10.3332/ecancer.2014.410DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963706PMC
April 2014
23 Reads
4 Citations

Publication Analysis

Top Keywords

cell lines
16
cell type
12
association brms1
12
brms1
12
largest tumour
8
brms1 immunostaining
8
brms1 mrna
8
location largest
8
tumour location
8
brms1 protein
8
mrna human
8
tumour dimension
8
tumour cell
8
suppressor brms1
8
protein expression
8
metastasis suppressor
8
human cell
8
uveal melanoma
8
tumour
7
cell
7

Similar Publications