Inhibition of hepatitis B virus cccDNA by siRNA in transgenic mice.

Cell Biochem Biophys 2014 Jul;69(3):649-54

Department of Clinical Laboratory, The Affiliated First Hospital of Harbin Medical University, Harbin, 150001, China.

The elimination of viral covalently closed circular DNA (cccDNA) from the nucleus of infected hepatocytes is an obstacle to achieving sustained viral clearance during antiviral therapy of chronic hepatitis B virus (HBV) infection. The aim of our study was to determine whether treatment with siRNA is able to suppress viral cccDNA amplification using a HBV-transgenic mice model. The experimental results revealed that siRNAs can serve as efficient alternative anti-HBV agents, because they showed better inhibitory effect on viral replication and antigen expression in transgenic mice. More importantly, the siRNA markedly inhibited HBV cccDNA amplification.

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http://dx.doi.org/10.1007/s12013-014-9847-1DOI Listing
July 2014
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