Crotalus durissus collilineatus Venom Induces TNF- α and IL-10 Production in Human Peripheral Blood Mononuclear Cells.

ISRN Inflamm 2014 19;2014:563628. Epub 2014 Jan 19.

Departamento de Biomedicina e Farmácia, Pontifícia Universidade Católica de Goiás, 74605-140 Goiania, GO, Brazil.

Snake venom has been the subject of numerous studies in an attempt to find properties and biological effects that may be beneficial to man. In this study we evaluated in vitro the effects of Crotalus durissus terrificus (Cdt) and Crotalus durissus collilineatus (Cdc) venom in human peripheral blood mononuclear cells (PBMCs). At 24 h, a significant decrease of viable cells was observed in cells stimulated with the Cdc venom at 0.0005 mg/mL and 0.005 mg/mL compared to the negative control. At 48 h, a significant decrease of viable cells was observed only in cells stimulated with Cdc venom at 0.005 mg/mL. A significant increase of TNF- α and IL-10 was detected 48 hours after culture of PBMC with Cdc, but not with Cdt venom. The expression of CD69 and PD1 (programmed death-1), activation and regulatory cell markers, on CD8+ and CD8- T cells did not change in the presence of Cdt and Cdc venom. Our results suggest the presence of proinflammatory and anti-inflammatory components in the Cdc venom. Further analysis should be done to identify those Cdc venom components as it has been done for the Cdt venom by other authors, indicating that modulatory components are found in the venom of different species of Crotalus snakes.

Download full-text PDF

Source
http://dx.doi.org/10.1155/2014/563628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3915804PMC
February 2014

Publication Analysis

Top Keywords

cdc venom
24
crotalus durissus
12
venom
11
decrease viable
8
viable cells
8
cdt venom
8
cells stimulated
8
peripheral blood
8
blood mononuclear
8
mononuclear cells
8
human peripheral
8
tnf- il-10
8
durissus collilineatus
8
stimulated cdc
8
observed cells
8
cells observed
8
cdc
7
cells
7
activation regulatory
4
24 h decrease
4

Similar Publications