A portable centrifugal analyser for liver function screening.

Authors:
Charles E Nwankire
Charles E Nwankire
Dublin City University
Ireland
Monika Czugala
Monika Czugala
Dublin City University
Ireland
Robert Burger
Robert Burger
University of California
United States
Kevin J Fraser
Kevin J Fraser
Dublin City University
Thomas Glennon
Thomas Glennon
Biomedical Diagnostics Institute
Blessing E Onwuliri
Blessing E Onwuliri
Abia State University Teaching Hospital
Nigeria
Isikaku E Nduaguibe
Isikaku E Nduaguibe
Abia State University Teaching Hospital

Biosens Bioelectron 2014 Jun 27;56:352-8. Epub 2014 Jan 27.

Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Ireland; School of Physical Sciences, Dublin City University, Ireland.

Mortality rates of up to 50% have been reported after liver failure due to drug-induced hepatotoxicity and certain viral infections (Gao et al., 2008). These adverse conditions frequently affect HIV and tuberculosis patients on regular medication in resource-poor settings. Here, we report full integration of sample preparation with the read-out of a 5-parameter liver assay panel (LAP) on a portable, easy-to-use, fast and cost-efficient centrifugal microfluidic analysis system (CMAS). Our unique, dissolvable-film based centrifugo-pneumatic valving was employed to provide sample-to-answer fashion automation for plasma extraction (from finger-prick of blood), metering and aliquoting into separate reaction chambers for parallelized colorimetric quantification during rotation. The entire LAP completes in less than 20 min while using only a tenth the reagent volumes when compared with standard hospital laboratory tests. Accuracy of in-situ liver function screening was validated by 96 separate tests with an average coefficient of variance (CV) of 7.9% compared to benchtop and hospital lab tests. Unpaired two sample statistical t-tests were used to compare the means of CMAS and benchtop reader, on one hand; and CMAS and hospital tests on the other. The results demonstrate no statistical difference between the respective means with 94% and 92% certainty of equivalence, respectively. The portable platform thus saves significant time, labour and costs compared to established technologies, and therefore complies with typical restrictions on lab infrastructure, maintenance, operator skill and costs prevalent in many field clinics of the developing world. It has been successfully deployed to a centralised lab in Nigeria.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2014.01.031DOI Listing
June 2014
24 Reads
8 PubMed Central Citations(source)
6.41 Impact Factor

Publication Analysis

Top Keywords

function screening
8
liver function
8
tests
4
tests accuracy
4
accuracy in-situ
4
laboratory tests
4
hospital laboratory
4
reagent volumes
4
tenth reagent
4
volumes compared
4
compared standard
4
standard hospital
4
in-situ liver
4
validated separate
4
79% compared
4
compared benchtop
4
benchtop hospital
4
hospital lab
4
variance 79%
4
coefficient variance
4

Similar Publications

Centrifugo-pneumatic multi-liquid aliquoting - parallel aliquoting and combination of multiple liquids in centrifugal microfluidics.

Lab Chip 2015 Aug;15(15):3250-8

Laboratory for MEMS Applications, IMTEK - Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110, Freiburg, Germany.

The generation of mixtures with precisely metered volumes is essential for reproducible automation of laboratory workflows. Splitting a given liquid into well-defined metered sub-volumes, the so-called aliquoting, has been frequently demonstrated on centrifugal microfluidics. However, so far no solution exists for assays that require simultaneous aliquoting of multiple, different liquids and the subsequent pairwise combination of aliquots with full fluidic separation before combination. Read More

View Article
August 2015

Fluidic automation of nitrate and nitrite bioassays in whole blood by dissolvable-film based centrifugo-pneumatic actuation.

Sensors (Basel) 2013 Aug 26;13(9):11336-49. Epub 2013 Aug 26.

Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.

This paper demonstrates the full centrifugal microfluidic integration and automation of all liquid handling steps of a 7-step fluorescence-linked immunosorbent assay (FLISA) for quantifying nitrate and nitrite levels in whole blood within about 15 min. The assay protocol encompasses the extraction of metered plasma, the controlled release of sample and reagents (enzymes, co-factors and fluorescent labels), and incubation and detection steps. Flow control is implemented by a rotationally actuated dissolvable film (DF) valving scheme. Read More

View Article
August 2013

Label-free impedance detection of cancer cells from whole blood on an integrated centrifugal microfluidic platform.

Biosens Bioelectron 2015 Jun 30;68:382-389. Epub 2014 Dec 30.

Biomedical Diagnostics Institute, National Center for Sensor Research, Dublin City University, Ireland; School of Physical Sciences, Dublin City University, Ireland. Electronic address:

An electrochemical Lab-on-a-Disc (eLoaD) platform for the automated quantification of ovarian cancer cells (SKOV3) from whole blood is reported. This centrifugal microfluidic system combines complex sample handling, i.e. Read More

View Article
June 2015

Centrifugo-pneumatic valving utilizing dissolvable films.

Lab Chip 2012 Aug 13;12(16):2894-902. Epub 2012 Jun 13.

Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland.

In this article we introduce a novel technology that utilizes specialized water dissolvable thin films for valving in centrifugal microfluidic systems. In previous work (William Meathrel and Cathy Moritz, IVD Technologies, 2007), dissolvable films (DFs) have been assembled in laminar flow devices to form efficient sacrificial valves where DFs simply open by direct contact with liquid. Here, we build on the original DF valving scheme to leverage sophisticated, merely rotationally actuated vapour barriers and flow control for enabling comprehensive assay integration with low-complexity instrumentation on "lab-on-a-disc" platforms. Read More

View Article
August 2012