A hard-wired glutamatergic circuit pools and relays UV signals to mediate spectral preference in Drosophila.

Authors:
Dr. Thangavel Karuppudurai, PhD
Dr. Thangavel Karuppudurai, PhD
National Institutes of Health (NIH)
Bethesda, Maryland | USA
Tzu-Yang Lin
Tzu-Yang Lin
National Health Research Institutes
Taiwan
Randall Pursley
Randall Pursley
National Institutes of Health
United States
Krishna V Melnattur
Krishna V Melnattur
University of Massachusetts
United States
Fengqiu Diao
Fengqiu Diao
National Institute of Mental Health
United States
Benjamin H White
Benjamin H White
National Institute of Mental Health
United States
Lindsey J Macpherson
Lindsey J Macpherson
The Scripps Research Institute
United States

Neuron 2014 Feb;81(3):603-615

Section on Neuronal Connectivity, Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Unlabelled: Many visual animals have innate preferences for particular wavelengths of light, which can be modified by learning. Drosophila's preference for UV over visible light requires UV-sensing R7 photoreceptors and specific wide-field amacrine neurons called Dm8. Here we identify three types of medulla projection neurons downstream of R7 and Dm8 and show that selectively inactivating one of them (Tm5c) abolishes UV preference. Using a modified GRASP method to probe synaptic connections at the single-cell level, we reveal that each Dm8 neuron forms multiple synaptic contacts with Tm5c in the center of Dm8's dendritic field but sparse connections in the periphery. By single-cell transcript profiling and RNAi-mediated knockdown, we determine that Tm5c uses the kainate receptor Clumsy to receive excitatory glutamate input from Dm8. We conclude that R7s→Dm8→Tm5c form a hard-wired glutamatergic circuit that mediates UV preference by pooling ∼16 R7 signals for transfer to the lobula, a higher visual center.

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A hard-wired glutamatergic circuit pools and relays UV signals to mediate spectral preference in Drosophila. Neuron, 81(3): 603-615 (Video Abstract). Recommended in F10

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http://dx.doi.org/10.1016/j.neuron.2013.12.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3920195PMC

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February 2014
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28 PubMed Central Citations(source)
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